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Efficient Screening Techniques to Identify Mutants with TR4 Resistance in Banana
The book consists of 11 chapters for mutation breeding and Fusarium wilt resistance screening in banana.
2023
Agriculture and Biotechnology Laboratories Activities Report 2021
Agriculture and Biotechnology Laboratories Activities Report 2021
2022
Library Preparation for Medium-to High-Throughput DNA Sequencing on the Illumina Sequencing Platform. A Laboratory Protocol
This protocol achieves parallel, cost-effective preparation of Illumina sequencing libraries using only standard laboratory equipment. Costs are less than $10 per sample, and the intended scale is dozens to hundreds of samples. End-products are sequencing libraries, where each library is individually indexed with a molecular barcode. These libraries can be pooled for sequencing and subsequently demultiplexed in-silico. We provide 24 forward and 36 reverse primer sequences with a combinatorial barcoding capacity for up to 864 samples.
2022
A Software Workflow for Automated Initial Analysis of High-throughput DNA Sequencing Project. A Laboratory Protocol
This protocol describes the use of a flexible Snakemake software workflow for reproducible analysis of genome re-sequencing experiments. It confirms sample relationships from the raw sequencing reads with alignment-free methods (kwip and mash) and performs alignment, variant calling, and variant annotation against one or several reference genomes; read alignments with bwa and/or NextGenMap, variant calling with freebayes and/or bcftools mpileup. If a genome annotation is available, variants are annotated with snpEff.
2022
Scientific Papers 2022
BOHRA, A., KILIAN, B., SIVASANKAR, S., CACCAMO, M., MBA, C., MCCOUCH, S.R., VARSHNEY, R.K. (2021) Reap the crop wild relatives for breeding future crops. Trends in Biotechnology Oct 8; S0167-7799(21)00197-9. RAHMAN, M.H., SULTANA, R., SARKAR, M.M.A., ISLAM, S., AZAD, M.A.K., SIVASANKAR, S. (2021) Comparative Profitability and Impact of BINA Developed Aman Mutant Rice Binadhan-7 with Non-Mutant Variety in Bangladesh. Asian Research Journal of Agriculture 14(3): 11-25. HUSSAIN, M., JANKULOSKI, L., HABIB-UR-RAHMAN, M., MALEK, M., KAMRUL ISLAM, Md., RAHEEMI, M.R., DANA, J., MYAT LWIN, K., AHMAD, F., RIZWAN, M., MOHYUDDIN TALHA, G., ASIF, M., ALI, S. (2021) Improving Sustainable Cotton Production Through Enhanced Resilience to Climate Change Using Mutation Breeding. In: Mutation Breeding, Genetic Diversity and Crop Adaptation to Climate Change. S. Sivasankar et al. (eds.), CABI. SOFKOVA-BOBCHEVA, S., PANTCHEV, I., KIRYAKOV, I., CHAVDAROV, P., MUHOVSKI, Y., SARSU, F., TOMLEKOVA, N. (2021) Induced Mutagenesis for Improvement of Bean (Phaseolus vulgaris L.) Production in Bulgaria. In: Mutation Breeding, Genetic Diversity and Crop Adaptation to Climate Change. S. Sivasankar et al. (eds.), CABI. JANKULOVSKA, M., IVANOVSKA, S., JANKULOSKI, L., MARKOSKI, M., KUZMANOVSKA, B., BOSHEV, D. (2021) Evaluation of Advanced Wheat Mutant Lines for Food and Feed Quality. In: Mutation Breeding, Genetic Diversity and Crop Adaptation to Climate Change. S. Sivasankar et al. (eds.), CABI. CéSPEDES, R., ARRIETA, N., BARQUERO, M., ABDELNOUR, A., NIELEN, S., INGELBRECHT, I. (2021) Determination of Radiosensitivity of Coffea arabica var. Venecia Seeds to Gamma-ray Irradiation. In: Mutation Breeding, Genetic Diversity and Crop Adaptation to Climate Change. S. Sivasankar et al. (eds.), CABI. 320–325. RABEFIRAISANA, H.J., GHANIM, A.M.A., ANDRIANJAKA, A., RASOAMAMPIONONA, B., JANKULOSKI, L., RAZAFINDRASOA, M.A., RAVELONJANAHARY, N.H., RAKOTOARISOA, N.V. (2021) Impact of Mulch-Based Cropping Systems Using Green Mulch and Residues on the Performance of Advanced Mutants Lines of Maize (Zea mays (L.)) Under Infested Field with the Parasitic Weed Striga asiatica (L.) Kuntze in Madagascar. In: Mutation Breeding, Genetic Diversity and Crop Adaptation to Climate Change. S. Sivasankar et al. (eds.), CABI. 235–242. JANKOWICZ-CIESLAK, J., GOESSNITZER, F., DATTA, S., VILJOEN, A., INGELBRECHT, I., TILL, B.J. (2021) Induced Mutations for Generating Bananas Resistant to Fusarium Wilt Tropical Race 4. In: Mutation Breeding, Genetic Diversity and Crop Adaptation to Climate Change. S. Sivasankar et al. (eds.), CABI. 366–378. THUDI, M., PALAKURTHI, R. SCHNABLE, J.C., CHITIKINENI, A., DREISIGACKER, S., MACE, E., SRIVASTAVA, R.K., SATYAVATHI, C.T., ODENY, D., TIWARI, V.K., LAM, H.M., HONG, Y.B., SINGH, V.K., LI, G., XU, Y., CHEN, X., KAILA, S., NGUYEN, H., SIVASANKAR, S., JACKSON, S.A., CLOSE, T.J., SHUBO, W., VARSHNEY, R.K. (2021) Genomic Resources in Plant Breeding for Sustainable Agriculture. Journal of Plant Physiology (TSI), 257. pp. 1–18. ISSN 0176-1617.?https://doi.org/10.1016/j.jplph.2020.153351 MATOVA, P.M., KAMUTANDO, C.N., SARSU, F., MAGOROKOSHO, C., LABUSCHAGNE, M. (2021) Determining the Optimum Gamma Irradiation Dose for Developing Novel Maize Genotypes. Journal of Crop Improvement. https://doi.org/10.1080/15427528.2020.1850591 SARSU, F., BIMPONG, I.K., JANKULOSKI, L. (2021). Contribution of Induced Mutation in Crops to Global Food Security. ACI Avences en Ciencias e Ingenierías, 12(22), 2–11. https://revistas.usfq.edu.ec/index.php/avances/issue/view/153
2022
Plant Breeding and Genetics Newsletter, No. 48, January 2022
Plant Breeding and Genetics Newsletter, No. 48, January 2022
2022
A Low-Cost Genotyping Protocol and Kit for Marker-Assisted Selection of Orange Lemma (rob1.a), a Feed Quality Trait in Barley (Hordeum Vulgare L.)
A Low-Cost Genotyping Protocol and Kit for Marker-Assisted Selection of Orange Lemma (rob1.a), a Feed Quality Trait in Barley (Hordeum Vulgare L.).
2021
Plant Breeding and Genetics Newsletter, No. 47, July 2021
Plant Breeding and Genetics Newsletter, No. 47, July 2021
2021
Development of Tolerant Crop Cultivars for Abiotic Stresses to Increase Food Security
Salt stress is the second most widespread soil problem after drought in arable crop production worldwide. Excess salt in soil adversely affects plant growth, development, and productivity when osmotic stress reduces water uptake by roots. This publication provides standard operating procedures for managing crop phenotyping in controlled/semi-controlled environments and natural field conditions. As a comprehensive reference, the publication will support IAEA Member States in the application of these procedures to increase the productivity of arable food crops to help withstand the impact of climate change on agriculture.
2021
Plant Breeding and Genetics Newsletter, No. 46, January 2021
Plant Breeding and Genetics Newsletter, No. 46, January 2021
2021
Agriculture and Biotechnology Laboratories Activities Report 2020
Agriculture and Biotechnology Laboratories Activities Report 2020
2020
Plant Breeding and Genetics Newsletter, No. 45, July 2020
Plant Breeding and Genetics Newsletter, No. 45, July 2020
2020
Plant Breeding and Genetics Scientific Papers 2020
Plant Breeding and Genetics Scientific Papers 2020
2020
Plant Breeding and Genetics Newsletter, No. 44, January 2020
Plant Breeding and Genetics Newsletter, No. 44, January 2020
2020
Agriculture and Biotechnology Laboratories Activities Report 2019
Agriculture and Biotechnology Laboratories Activities Report 2019
2019