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          Isotopes to Study Nitrogen Pollution and Eutrophication of Rivers and Lakes

          Closed for Proposals

          Project Type

          Coordinated Research Project

          Project Code

          F32007

          CRP

          2134

          Approved Date

          12 May 2015

          Project Status

          Closed

          Start Date

          22 February 2016

          Expected End Date

          21 February 2020

          Completed Date

          12 December 2022

          Participating Countries

          Cuba

          Description

          Water quality in thousands of lakes and rivers around the globe is deteriorating due to rising nutrient levels and other chemical pollutants mainly of anthropogenic origin (e.g., agriculture, sewage, industrial discharges, urban areas) causing changes in their ecological structure and function. The excessive nitrogen pollution (nitrate, ammonium) of surface waters has resulted in change of oligotrophic water bodies to eutrophic and sometimes to hypertrophic states. Environmental isotopes are used not only to assess hydrological processes in terms of surface water quantity, but also to address water quality issues, such as nitrogen pollution. This CRP aims to improve the suite of isotopic markers combined with other chemical / biological substances to better define the possible sources and re-cycling of nitrogen pollution in surface water bodies. The CRP aims to deepening the knowledge on the temporal (diel, seasonal) and in-depth variation of nitrogen isotope data, combined with additional substances, as deeper water bodies may sometimes prevent a complete understanding of nitrogen isotope dynamics from surface water collections. Moreover, improving conventional and promoting new methodologies on nitrogen species isotopic characteristics is expected to facilitate access to a more routine and low-cost use of nitrogen and oxygen isotope data of nitrogen species. Overall, the CRP will aim at improving the capability and expertise among participating Member States in the use of environmental isotopes to better assess the impact of nitrogen pollution and eutrophication on surface water resources availability and sustainability.

          Objectives

          To improve capability and expertise among Member States in the use environmental isotopes to better assess impacts of nitrogen pollution on water resources variability, availability and sustainability.

          Specific Objectives

          Assess and improve the interpretation of hydrological processes, sources, interactions and pathways in rivers and lakes.

          Assess and improve the suite of isotopes and other chemical and/or biological indicators to evaluate the environmental and anthropogenic impact on surface waters in terms of identifying more accurately the multiple sources of nitrogen pollution and eutrophication.

          Assess and improve the understanding and interpretation of N-nutrient dynamics, eutrophication and sediment transport in lakes and rivers.

          Explore improved analytical methods of N-related isotope parameters in order to facilitate access to isotope data of nitrogen species and build greater confidence in assessments of nitrogen pollution issues of surface waters.

          Impact

          The project had a significant impact in the participating countries. In 7 countries it was the first time that N-isotopes were ever applied to assess impact of nitrogen pollution on water resources variability, availability and sustainability. New and young researchers have benefited from their involvement in the project (e.g. Malaysia) though Master’s and PhD theses and were introduced on the use of isotope hydrology techniques. The participants were able to publish their findings in conferences and peer-reviewed journals, which is ample evidence of the improved capability and expertise in the use of environmental isotopes to better assess impacts of nitrogen pollution on water resources variability, availability and sustainability, and which is expected to help the stakeholders adopt protective measures and sound remediation approaches. A large dataset of nitrate isotopes was introduced in GNIR database and is available to Member States. Scientifically-wise, the project offered the identification of global patterns related to nitrate isotopes, which were linked to nitrate sources and biogeochemical processes driven by climatic and meteorological factors. For example, the project showed that river waters undergo quality deterioration due to nitrogen contamination originating mainly from organic nitrogen in manure and septic wastes (46%) and less from dissolved organic matter in the soil (28%) and the application of mineral fertilizers (21%). The link between N cycling and climate was documented through a global survey, and is expected to help further investigate climate change impact on nitrogen cycle. The perception of the locals concerning the water scarcity and contamination was examined through a socio-hydrogeological survey, which indicated “Supply clean water to public” as the main objective of water management in Sri Lanka.

          Relevance

          The scientific and hydrological results of the CRP clearly demonstrated that the recent developments in field (simpler sampling protocols) and laboratory (use of cheaper, easier to use titanium chloride sample preparation and use of laser absorption spectrometers) methods allow the routine use of this tracer to assess nitrate pollution in a variety of hydrological and climatic settings. The CRP has developed SOP for both field and laboratory methods, ready to be transferred to counterparts involved in both CRPs and TC projects.

          CRP Publications

          Isotope Bioscience Laboratory - ISOFYS, Department of Green Chemistry and Technology, Faculty of Bioscience Engineering, Ghent University, Gent, Belgium
          Journal Article
          2019
          Land use controls Kenyan riverine nitrate discharge into Lake Victoria - Evidence from Nyando, Nzoia and Sondu Miriu river catchments
          Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, China; Institute of Surface-Earth System Science, Tianjin University, Tianjin, China
          Journal Article
          2018
          Spatial variation of nitrogen cycling in a subtropical stratified impoundment in southwest China, elucidated by nitrous oxide isotopomer and nitrate isotopes
          Centre for Climate and Environmental studies, Indian Institute of Science Education and Research Kolkata, India
          Journal Article
          2020
          Spatial distribution of d18O values of water in the Ganga river basin: Insight into the hydrological processes
          Graduate School of Nuclear and Allied Sciences, Atomic Energy Commission, Accra, Ghana
          Journal Article
          2020
          Nitrate contamination and source apportionment in surface and groundwater in Ghana using dual isotopes (15N and 18O-NO3) and a Bayesian isotope mixing model
          School of Industrial Technology, Universiti Sains Malaysia, Malaysia
          Journal Article
          2020
          Nitrate sources and processes in the surface water of a tropical reservoir by stable isotopes and mixing model
          Universidad de Concepción, Chile
          Journal Article
          2022
          An Analysis of the Effects of Large Wildfires on the Hydrology of Three Small Catchments in Central Chile Using Tritium-Based Measurements and Hydrological Metrics
          British Geological Survey (BGS), Maclean Building, Wallingford, UK
          Journal Article
          2020
          Determining the impact of riparian wetlands on nutrient cycling, storage and export in permeable agricultural catchments
          Centro de Estudios Ambientales de Cienfuegos (CEAC), Cuba
          Report
          2020
          Nitrogen and Oxygen isotopes of dissolved nitrates, results obtained from the first experience in Cuba
          University of Eastern Finland, Kuopio, Finland
          Chapter
          2020
          The effect of Yedoma soils on nitrogen dynamics in the Lena River
          British Geological Survey (BGS), Maclean Building, Wallingford, UK
          Journal Article
          2020
          Seasonal variability of sediment controls of nitrogen cycling in an agricultural stream
          Aristotle University of Thessaloniki, School of Geology, Thessaloniki, Greece
          Journal Article
          2020
          Origin, implications and management strategies for nitrate pollution in surface and ground waters of Anthemountas basin based on a d15N-NO3- and d18O-NO3- isotope approach
          British Geological Survey (BGS), Maclean Building, Wallingford, UK
          Journal Article
          2017
          Opening Opportunities for High-Resolution Isotope Analysis - Quantification of d15NNO3 and d18ONO3 in Diffusive Equilibrium in Thin–Film Passive Samplers
          USA
          Journal Article
          2021
          Nitrate ?17O tracer method for determining gross nitrification rates
          Applied Geology and Geo-Environment Laboratory, Faculty of Sciences, Ibn Zohr University, Morocco
          Chapter
          2018
          Isotopic and Chemical Tracers for the Sustainable Management of Water Resources in Semi-arid Area: Case of Massa Catchment, Morocco
          Universidad de Concepción, Chile
          Report
          2020
          Effect of transitory storage zone on Nitrogen dynamic. A case study from a heavily cropped territory in Chiles’ Central Valley
          IAEA
          Journal Article
          2021
          Global patterns of nitrate isotope composition in rivers and adjacent aquifers reveal reactive nitrogen cascading
          Centre for Climate and Environmental studies, Indian Institute of Science Education and Research Kolkata, India
          Report
          2020
          Nitrogen pollution in large river system: A case study in the river Ganga, India
          Kenya
          Conference
          2017
          Carbon and nutrients sources and transformation in the River Sabaki, Kenya
          Isotope Bioscience Laboratory - ISOFYS, Department of Green Chemistry and Technology, Faculty of Bioscience Engineering, Ghent University, Gent, Belgium
          Journal Article
          2020
          Tracking sources and fate of groundwater nitrate in Kisumu city and Kano plains, Kenya
          Italyian counterpart
          Report
          2020
          Squaring the cycle: the INTEgration of GROundwater processes in Nutrient budgets for a basin-oriented remediation strategy (INTEGRON)
          Department of Earth Sciences, University of Pisa, and Department of Earth and Environmental Sciences, University of Pavia, Italy
          Journal Article
          2021
          A critical assessment of widely used techniques for nitrate source apportionment in arid and semi-arid regions
          School of Industrial Technology, Universiti Sains Malaysia, Malaysia
          Journal Article
          2018
          Application of Nitrate Stable Isotopes, d15N-NO3- and d18O-NO3-, in Bukit Merah Reservoir, Malaysia
          Isotope Hydrology Section, Sri Lanka Atomic Energy Board, Orugodawatta, Wellampitiya, Sri Lanka
          report
          2020
          Isotope Investigation on Eutrophication of Rivers and Lakes under Stressed Conditions; Lake Gregory and River Kelani in Sri Lanka
          IAEA
          Conference
          2020
          A global synthesis of dual nitrate isotope values in rivers and groundwaters
          Department of Geography and Environmental Studies, Wilfrid Laurier University, Waterloo, Ontario, Canada
          Journal Article
          2019
          Quantifying the Fate of Wastewater Nitrogen Discharged to a Canadian River
          IAEA
          Journal Article
          2019
          A Ti(III) reduction method for one-step conversion of seawater and freshwater nitrate into N2O for stable isotopic analysis of 15N/14N, 18O/16O and 17O/16O
          Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, China; Institute of Surface-Earth System Science, Tianjin University, Tianjin, China
          Journal Article
          2018
          Nitrate dynamics during impoundment and flood periods in a subtropical karst reservoir: Hongfeng Lake, Southwestern China
          Institute of Chemistry, Vietnam Academy of Science and Technology, Vietnam
          Journal Article
          2020
          Stable isotopes as an effective tool for N nutrient source identification in a heavily urbanized and agriculturally intensive tropical lowland basin
          Instituto de Investigaciones Marinas y Costeras, Consejo Nacional de Investigaciones Científicas y Técnicas (UNMdP-CONICET), FCEyN, Argentina
          Journal Article
          2020
          A biological and nitrate isotopic assessment framework to understand eutrophication in aquatic ecosystems
          Applied Geology and Geo-Environment Laboratory, Faculty of Sciences, Ibn Zohr University, Morocco
          Journal Article
          2019
          Assessment of water quality and nitrate source in the Massa catchment (Morocco) using d15N and d18O tracers

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