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          Mutation Breeding for Resistance to Striga Parasitic Weeds in Cereals for Food Security

          Closed for proposals

          Project Type

          Coordinated Research Project

          Project Code

          D25005

          CRP

          2166

          Approved Date

          9 December 2015

          Status

          Closed

          Start Date

          6 May 2016

          Expected End Date

          5 May 2022

          Completed Date

          23 June 2021

          Participating Countries

          Burkina Faso
          China
          Ethiopia
          Iran (Islamic Republic of)
          Italy
          Japan
          Kenya
          Kingdom of the Netherlands
          Madagascar
          Sudan
          Türkiye
          United States of America

          Description

          The parasitic weeds Striga asiatica and S. hermonthica are major biological constraints to cereal production in most of sub-Saharan Africa and semi-arid tropical regions of Asia. Yield losses are mainly in maize, millet, rice and sorghum and most severe on marginal lands of subsistence farmers. The unique biology of Striga, tightly adapted to its crop hosts, limits control options. Because subsistence farmers either cannot afford or do not have access to inputs, host plant resistance is a vital part of Striga control. Resistance traits, however, are lacking in most cereal varieties sown in Striga prone areas. This CRP proposes the use of physical mutagenesis and associated screening technologies to broaden the genetic base of resistance. The project seeks to link cereal improvement programs in the Member States with Striga biologists and IAEA staff. This would combine expertise in screen house?and laboratory screening to enhance mutation breeding to timely develop or adapt screening packages to generate novel sources of Striga resistance. Initial focus will be on sorghum and rice because of their importance to food security and amenability to mutation breeding techniques. Up to ten research contracts are expected to be awarded and five no-cost agreement holders from advanced laboratories and research institutes with recognized expertise in the targeted technologies will be invited to share their experience with the contract holders and contribute to the development and validation of the planned technical packages. In addition, it is foreseen that two technical contracts will be awarded for services in advanced areas such as marker development and advanced efficiency enhancing technologies and mutant characterization. Coordination and technical management will be handled by the scientific secretary in the Plant Breeding and Genetics Laboratory/Section.

          Objectives

          To support generation of novel sources of variation, using mutation breeding, by developing efficient screening protocols for Striga resistance in cereals for building research capacity and improving food security in Member States.

          Specific objectives

          To develop, optimize, validate and disseminate robust screening protocols for mutant populations for resistance/tolerance to the parasitic weed Striga in major cereal crops.

          To integrate and adapt efficiency enhancing techniques in the mutation breeding processes.

          To generate genetic diversity to develop resistant/tolerant varieties to Striga infestation.

          To improve capacity in efficient mutation breeding for resistance to parasitic weeds in cereals.

          To develop, optimize, validate and disseminate robust screening protocols for mutant populations for resistance/tolerance to the parasitic weed Striga in major cereal crops.

          To integrate and adapt efficiency enhancing techniques in the mutation breeding processes.

          To generate genetic diversity to develop resistant/tolerant varieties to Striga infestation.

          To improve capacity in efficient mutation breeding for resistance to parasitic weeds in cereals.

          Impact

          The CRP improved human capacity in mutation breeding and efficiency enhancing technologies in Striga affected members states, and the developed resistant mutant lines in the major cereals (sorghum, rice and maize) are expected to generate impact on sustainable production and food security particularly in the Striga affect countries in Africa. FAO estimates that annual crop loss due to Striga across Africa exceeds US $7 billion, impacting over 300 million people and up to 50 million hectares of crop land are infested with Striga. The developed Striga resistant varieties in the CRP will contribute significantly to reduce the loss due to the Striga across Africa and else where.

          Relevance

          The CRP is of typical relevance to the nuclear application for food and agriculture. The results generated are of relevance to integrated management of Striga parasitic weeds in cereals in affected countries mostly in Africa and part of Asia. Further more the outputs of the CRP are important in ensuring food security specially in the era of climate change as drought and consequently the effect of Striga is expected to expand and intensify. In addition, the advanced information and approach accumulated from CRP could be of relevance to scientific research related to plant-pathogen interactions.

          CRP Publications

          Type

          peer reviewed

          Year

          2020

          Publication URL

          https://advances.sciencemag.org/content/6/44/eabc2385

          Description

          Ethylene signaling mediates host invasion by parasitic plants

          Country/Organization

          Japan

          Type

          Conference paper

          Year

          2018

          Publication URL

          https://inis.iaea.org/search/search.aspx?orig_q=RN%3A50011058

          Description

          Enhancing Efficiency of Mutation Breeding for Striga Resistance in Sorghum by Haploid Technology

          Country/Organization

          Iran/PBGL

          Type

          Conference paper

          Year

          2019

          Publication URL

          https://community.plantae.org/article/5139847806114072186/plant-cells-amp-tissue…

          Description

          Improvement of embryogenesis in anther versus shed microspore culture of rice (Oryza sativa L.)

          Country/Organization

          Iran/PBGL

          Type

          Peer reviewed

          Year

          2019

          Publication URL

          https://doi.org/?10.3389%2Ffpls.2019.00328

          Description

          Generation is indispensable for haustorium formation of the root parasitic plant Striga hermonthica

          Country/Organization

          Japan

          Type

          Conference paper

          Year

          2018

          Publication URL

          https://inis.iaea.org/search/search.aspx?orig_q=RN%3A50011056

          Description

          Haploidy in Rice (Oryza Sativa L.) Mutation Breeding for Striga Resistance

          Country/Organization

          Iran/PBGL

          Type

          Peer reviewed

          Year

          2019

          Publication URL

          https://www.sciencedirect.com/science/article/pii/S0960982219310103

          Description

          Genome sequence of Striga asiatica provides insight into the evolution of plant parasitism

          Country/Organization

          Japan

          Type

          Peer reviewed

          Year

          2021

          Publication URL

          https://pubmed.ncbi.nlm.nih.gov/33783524/

          Description

          Molecular dissection of haustorium development in Orobanchaceae parasitic plants.

          Country/Organization

          Japan

          Type

          Peer reviewed

          Year

          2019

          Publication URL

          http://www.plantphysiol.org/content/179/4/1796

          Description

          The Structural Integrity of Lignin Is Crucial for Resistance against Striga hermonthica Parasitism in Rice.

          Country/Organization

          Japan/Kenya

          Type

          Peer reviewed

          Year

          2020

          Publication URL

          https://academic.oup.com/plphys/article/185/4/1429/6119653

          Description

          Three-dimensional reconstructions of the internal structures of haustoria in parasitic Orobanchaceae.

          Country/Organization

          Japan

          Type

          Peer reviewed

          Year

          2019

          Publication URL

          https://www.frontiersin.org/articles/10.3389/fpls.2019.01056/full

          Description

          Haustorium Inducing Factors for Parasitic Orobanchaceae

          Country/Organization

          Japan

          Type

          FAO/IAEA Agriculture and Biotechnology Laboratories - NAFA Activities report

          Year

          2019

          Publication URL

          http://www-naweb.iaea.org/nafa/resources-nafa/ABL-Activities-Report-2019.pdf

          Description

          Validated glasshouse and laboratory protocols for confirmation of Striga-resistant cereal mutants and identification of underlying resistance mechanism

          Country/Organization

          PBGL Seibersdorf

          Type

          Web-based success story

          Year

          2019

          Publication URL

          http://www.dgdingfa.net/newscenter/news/nuclear-techniques-help-develop-new-sorghum…

          Description

          Nuclear Techniques Help Develop New Sorghum Lines Resistant to the Parasitic Weed Striga

          Country/Organization

          FAO/IAEA Joint Center for NA

          Type

          peer reviewed

          Year

          2018

          Publication URL

          https://pubmed.ncbi.nlm.nih.gov/29498051/

          Description

          Host lignin composition affects haustorium induction in the parasitic plants Phtheirospermum japonicum and Striga hermonthica

          Country/Organization

          Japan

          Type

          Peer reviewed paper

          Year

          2020

          Publication URL

          https://doi.org/10.4236/ajps.2020.1110112

          Description

          Induced Resistance to Striga hermonthica in Sorghum by Gamma Irradiation. American Journal of Plant Sciences, 11, 1545-1561.

          Country/Organization

          Burkina Faso/ PBGL ,Seibersdorf

          Type

          Conference paper

          Year

          2019

          Publication URL

          https://conferences.iaea.org/indico/event/145/contributions/5031

          Description

          Induced mutation in Sorghum for Striga-resistance

          Country/Organization

          Burkina Faso and PBGL

          Type

          peer reviewed

          Year

          2020

          Publication URL

          https://journals.biologists.com/dev/article/147/14/dev187781/224373/An-auxin-tra…

          Description

          Auxin transport network underlies xylem bridge formation between the hemi-parasitic plant Phtheirospermum japonicum and host Arabidopsis

          Country/Organization

          Japan

          Type

          Conference paper

          Year

          2019

          Publication URL

          https://conferences.iaea.org/indico/event/145/contributions/5031

          Description

          INDUCED MUTATION FOR DEVELOPING MUTANT RICE LINES TOLERANT TO THE PARASITIC WEED STRIGA ASIATICA

          Country/Organization

          Madagascar and PBGL

          Type

          Peer reviewed

          Year

          2018

          Publication URL

          https://doi.org/10.1242/dev.164848

          Description

          Induced cell fate transitions at multiple cell layers configure haustorium development in parasitic plants

          Country/Organization

          Japan

          Type

          Peer reviewed

          Year

          2020

          Publication URL

          https://webcache.googleusercontent.com/search?q=cache%3Ai5GltiAEjP8J%3Ahttps%3A/…

          Description

          Subtilase activity in the intrusive cells mediates haustorium maturation in parasitic plants

          Country/Organization

          Japan

          Type

          conference paper

          Year

          2018

          Publication URL

          https://inis.iaea.org/collection/NCLCollectionStore/_Public/50/009/50009355.pdf?…

          Description

          Mutation Breeding for Resistance to Striga Hermonthica (Del.) Benth. in Sorghum for Food Security in Burkina Faso (ID 204)

          Country/Organization

          Burkina Faso/PBGL

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