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          Climate Proofing of Food Crops: Genetic Improvement for Adaptation to High Temperatures in Drought Prone Areas and Beyond

          Closed for Proposals

          Project Type

          Coordinated Research Project

          Project Code

          D23029

          CRP

          1756

          Approved Date

          2 September 2010

          Project Status

          Closed

          Start Date

          15 February 2011

          Expected End Date

          15 February 2016

          Completed Date

          12 October 2016

          Description

          Climate change is now largely accepted as a real and pressing global problem. The Intergovernmental panel on Climate Change (IPCC) considers four families of socio-economic development and associated emission scenarios known as Special Report on Emissions Scenarios: A2, B2, A1, and B1. Out of these; A1, the ‘‘business-as-usual scenario,’’ corresponds to the highest emissions of greenhouse gases and aerosol precursors, and B1 corresponds to the lowest, the other scenarios being intermediate between these two. It has recently been estimated that developing countries will bear 70-80% of the costs of climate change damage with agriculture being the most impacted sector. The main impacts of climate change on agriculture will most probably be experienced through higher temperatures (increase in minima and maxima), altered changes in rainfall patterns (in amount, spatial and temporal distributions), increased rates of evaporation, increased intensity and frequency of extreme events (floods and droughts), and raise of sea level affecting coastal areas where large quota of cultivated land are located (intrusion of salty water).

          The responses that agriculture systems world wide can put in place to cope with the expected impact of climate change and to reduce the food insecurity range from institutional and policy levels to the best management practices and technology advancement. An important opportunity in terms of technology advancement is offered by the genetic improvement of crops that can adapt to the future climate conditions; i.e., “climate proofing” crops. Additionally, data collected from the selected crops: rice and common bean during the course of the CRP may be useful for a study of mathematical models for responses to high temperature in association with AquaCrop - FAO.

          This CRP will focus on improving the grain yields of a major cereal (rice) and a grain legume (common bean) to high temperature stress in the face of climate change. These two crops are essential staples in the diets of millions of impoverish and vulnerable populations, and therefore any attempt in increasing their yields under high temperature stress could have a major and positive impact in terms of food security, improved health and income generation. The intent is to develop tools that allow plant breeders using mutation programs together with enhancing plant biotechnologies to develop improved crop varieties with higher and wider adaptability to temperatures variations as according to SRES predictions. Up to 10 research contracts are expected to be awarded and four no cost agreement holders from advanced laboratories with solid knowledge in the selected technologies and fields of expertise invited to share their experience with the contract holders. In addition, it is foreseen that 2 technical contracts will be awarded for services in identification and isolation of mutant genes in model crops using high throughput DNA technologies. Coordination and technical management will be handled by the scientific secretary in the Plant Breeding and Genetics Section.

          Specific Objectives

          To explore whole plant genetic variability and identify high yielding genotypes from existing natural and mutated germplasm of cereals (preferably rice) and nitrogen fixing grain legumes (preferably common bean) for adaptation to high temperature; establishing robust experimental protocols for physiological, genetic and molecular studies; use advanced biotechnology, bioinformatics and genomics tools for whole plant analysis and data visualization.

          To facilitate technology transfer, sharing of genetic and knowledge resources (through electronic means, peer reviewed publications, workshops, training courses, field days) and foster networks between participating research groups and potential end users for their mutual benefit

          Impact

          (i) Development of hands on protocols and robust efficient screening techniques in rice that will help other scientist in MSs to fast screen mutant population/genotypes for heat stress temperature
          (ii) Development of new germplasm through mutation breeding and assisted biotechnologies in rice and common bean
          (iii) Release of new rice mutant variety with has better heat tolerance than local varieties
          (iv) Development of new mutant lines which are pipeline to release and contribute food security in MSs with better adaptation to climate change
          (v) Increased knowledge and experience to resilience for heat stress tolerance for current and future climate change
          (vi) Dissemination of research results through lectures to the students, proceeding/presentations in conferences/workshops and about 40 scientific publications (papers, book and book chapters)
          (vii) Dissemination of produced information under the CRP through outreach activities including trainings, farmers day, workshops, public exhibitions
          (viii) Integration between mutation breeding and molecular genetics scientists in this CRP developed bi- and multi-lateral collaborations and facilitated sharing genetic and knowledge resources in different platforms
          (ix) Productions pf PhD, MSc and BS thesis on research directly contributing to this CRP
          (x) Contribute food security through better adapted climate change varieties
          (xi) Enhanced R&D capacity on phenotypical, physiological and genotypical screening of mutant rice and bean for heat stress tolerance to better adapt on-going and future climate change scenarios
          (xii) Enhanced public awareness about the importance of potential of plant mutation breeding for meeting resilience of climate change and global warming

          Relevance

          Improving the delivery of knowledge /experience for meeting resilience of climate change and global warming.

          CRP Publications

          Jawaharlal Nehru University (JNU)-INDIA
          Oral Presentation International symposium on rice functional genomics -INDIA
          2013
          Das P, Singla---Pareek SL and Pareek A. (2013) Characterization of gamma-ray induced rice mutant reveals the alteration in plant morphology, chloroplast structure, thylakoid organization and expression of transcripts involved
          Universidad Nacional Autónoma de México
          Journal- BMC Genomics
          2012
          Pablo Peláez, Minerva S Trejo, Luis P I?iguez, Georgina Estrada-Navarrete, Alejandra A Covarrubias, José L Reyes and Federico Sanchez; Identification and characterization of microRNAs in Phaseolus vulgaris by high-throughput sequencing
          India
          Poster presentation /Inter Drought V,21-25 February 2017, Book of Abstracts, p156, abstract number 444.
          2017
          Pre-Field Screening for Heat Tolerant Mutants in Rice, Fatma Sarsu, Abdelbagi M.A. Ghanim, Priyanka Das, Rajeev Bahuguna, Kusolwa Paul Mbogo, Muhammad Ashraf, Sneh Lata Singla Pareek, Ashwani Pareek, Brian P. Forster, Ivan Ingelbrecht
          Centro Internacional de Agricultura Tropical (CIAT)- COLOMBIA
          Turkish Journal of Field Crops 18: 73-77.
          2013
          Ghanbari, A. A., S. H. Mousavi, A. M. Gorji and I. Rao. 2013. Effects of water stress on leaves and seeds of bean (Phaseolus vulgaris L.).
          Paper- Molecular Breeding. 34: 1765-1776
          2014
          Competitive amplification of differentially melting amplicons facilitates efficient genotyping of photoperiod- and temperature- sensitive genic male sterility in rice, Zhang H-L, Huang J-Z*, Chen X-Y, Tan Y-Y, Shu Q-Y*
          Cuba
          Cultivos Tropicales, 2016, vol. 37, no. 1 (enero-marzo)
          2016
          Selección de genotipos de arroz de buen comportamiento agronómico en condiciones de bajo suministro de agua .María C. González Cepero y Anirebis Martínez Romero .
          Pakistan
          Paper presented in the International Conference on Stress Biology and Biotechnology: Challenges & Management. Institute of Agricultural Sciences, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan. pp15.
          2014
          Screening for heat tolerance in rice (Oryza sativa L.) through morpho-physiological profiling, Zafar, S. A., Hameed, A., Ashraf, M. and Khan, A. S. 2014.
          Cuba
          Cultivos Tropicales, 2016, vol. 37, no. 1 (enero-marzo)
          2016
          Evaluación de cultivares de arroz en diferentes zonas de producción arrocera de “Los Palacios”, Pinar del Río, para su utilización en programas de mejoramiento .Noraida de J. Pérez León, María C. González Cepero, Rodolfo I. Castro Mendui?a y Manuel Aguilar.
          Centro Internacional de Agricultura Tropical (CIAT)- COLOMBIA
          Handbook of Plant and Crop Physiology. pp. 847-889. Pessarakli M., Eds. CRC Press, Taylor and Francis Group, USA.
          2014
          Rao, I. M. Advances in improving adaptation of common bean and Brachiaria forage grasses to abiotic stress in the tropics.
          Centro Internacional de Agricultura Tropical (CIAT)-COLOMBIA
          Turkish Journal of Field Crops 18: 73-77.
          2013
          Ghanbari, A. A., S. H. Mousavi, A. M. Gorji and I. Rao. 2013. Effects of water stress on leaves and seeds of bean (Phaseolus vulgaris L.).
          China
          Paper- -Southwest China Journal of Agricultural Sciences- 26:1387-1391
          2013
          Influences of Natural High-temperature Stress on Heat Resistance of Rice- Su P, Feng H, Chen H, Yang CM, Wu XB, Huang Q
          Austria, Vienna
          Poster presentation Plant Abiotic Stress III. Congress 29 June- 1 July 2015, Vienna, Austria. Book of Abstract page 48.
          2015
          Development of Heat Tolerance Screening Techniques of Common and Tepary Beans Mutants for Adaptation to High Temperature in Drought Prone Areas-Sarsu, F., Caridad M., and Mu?oz C.
          China
          Paper, Scientia Agricultura Sinica. 48(1): 1-9 (doi: 10.3864/j.issn.0578-1752.2015.01.001)
          2015
          Identification and transition analysis of photo- /thermo-sensitive genic male sterile genes in two-line hybrid rice in China, Zhang H-L, Chen X-Y, Huang J-Z, E Z-G, Gong J-Y, Shu Q-Y
          Centro Internacional de Agricultura Tropical (CIAT)-COLOMBIA
          Field Crops Research 148: 24-33.
          2013
          Assefa, T., S. E. Beebe, I. M. Rao, J. B. Cuasquer, M. C. Duque, M. Rivera, A. Battisti, M. Lucchin. 2013. Pod harvest index as a selection criterion to improve drought resistance in white pea bean.
          Centro Internacional de Agricultura Tropical (CIAT)- COLOMBIA
          Plant Soil 364: 29-37.
          2013
          Devi, J. M., T. R. Sinclair, S. E. Beebe and I. M. Rao. 2013. Comparison of common bean (Phaseolus vulgaris L.) genotypes for nitrogen fixation tolerance to soil drying.
          Centro Internacional de Agricultura Tropical (CIAT)-COLOMBIA
          Plant Soil 364: 29-37./Journal
          2013
          Devi, J. M., T. R. Sinclair, S. E. Beebe and I. M. Rao. 2013. Comparison of common bean (Phaseolus vulgaris L.) genotypes for nitrogen fixation tolerance to soil drying.
          China
          Paper-Molecular Breeding. 34: 481-489
          2014
          Characterization of an RNase Z nonsense mutation identified exclusively in environment-conditioned genic male sterile rice. Zhang H-L, Huang J-Z, Liu Q-L, Nawaz Z, Lu H-P, Gong J-Y, Zhu Y-J, Yan W, Shu Q-Y*
          Jawaharlal Nehru University (JNU)-INDIA
          Plant Signal Behav. 2012 Nov 3;8(1). pii: e22734.
          2012
          Kumari S, Roy S, Singh P, Singla-Pareek SL, Pareek A.; Cyclophilins: Proteins in search of function.
          Department of Biology/UK
          Plant biotechnology and agriculture
          2012
          Heslop-Harrison JS, Schwarzacher T. Genetics and genomics of crop domestication :Prospects for the 21st century.
          Centro Internacional de Agricultura Tropical (CIAT)-COLOMBIA
          Frontiers in Plant Physiology 4, 35 (1-20).
          2013
          Beebe, S., I. Rao, M. Blair and J. Acosta. 2013. Phenotyping common beans for adaptation to drought.
          Beijing, China
          The 7th International Crop Science Congress, August 14-19, 2016,
          2016
          Huang J-Z. Frequency and type of inheritable mutations induced by gamma rays in rice as revealed by whole genome sequencing.
          Centro Internacional de Agricultura Tropical (CIAT)-COLOMBIA
          Plant Soil 372: 3-25/Journal
          2013
          Yang, Z., I. M. Rao and W. J. Horst. 2013. Interaction of aluminium and drought stress on root growth and crop yield on acid soils.
          Bangkok, Thailand
          Poster presentation- 4th International Rice Congress, 27 October- 01 November 2014, Bangkok, Thailand. Book of Abstract, p 85
          2014
          Climate proofing of food crops: Genetic improvement for adaptation to high temperatures in drought prone areas and beyond. 4th International Rice Congress-SARSU, F., M., M., SPENCER, C. HAO, A. PAREEK, P., DAS and T., PADOLINA
          Universidad Nacional Autónoma de México
          Oral Presentation, National Congress, XV Congress of Plant Biochemistry and Molecular Biology.
          2012
          Peláez et al. 2012 ; A conserved bZIP mRNA involved in the Unfolded Protein Response (UPR) is processed during common vean root nodule ontogeny
          Centro Internacional de Agricultura Tropical (CIAT)- COLOMBIA
          African Crop Science Journal 21: 265-281.
          2013
          Rao, I., S. Beebe, J. Polania, J. Ricaurte, C. Cajiao, R. García and M. Rivera; Can tepary bean be a model for improvement of drought resistance in common bean?
          Jawaharlal Nehru University (JNU)- INDIA
          Oral Presentation/International symposium on rice functional genomics. Page 66.
          2013
          Roy S, Kumari S, Singh P, Singla---Pareek SL, Pareek A (2013). A highly abundant, stress regulated rice cyclophilin (OsCyp2) confers multistress tolerance to evolutionary diverse organisms.
          Pakistan
          Pak. J. Botany. Pak. J. Bot., 49(2): 453-463, 2017
          2017
          Heat shock induced morpho- physiological response in indica rice (Oryza sativa L.) at early seedling stage, Zafar, S. A., Hameed, A., Khan, A. S. and Ashraf, M. 2017.
          Jawaharlal Nehru University (JNU)- INDIA
          Wageningen academic publisher. The Netherlands. ISBN: 978-90-8686-234-4. (in press).
          2013
          Eds: Nasya Tomlekova, Imran Kozgar and Rafiq Wani; A Powerful Approach for Obtaining Abiotic Stress Tolerant Crops and Upgrading Food Security for Human Nutrition. In: Mutagenesis: exploring novel genes and pathways.
          Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
          Oral Presentation/ National Congress, XV Congress of Plant Biochemistry and Molecular Biology
          2012
          Peláez et al. 2012 . A conserved bZIP mRNA involved in the Unfolded Protein Response (UPR) is processed during common vean root nodule ontogeny”
          Austria, Vienna
          Poster presentation Plant Abiotic Stress III. Congress 29 June- 1 July 2015, Vienna, Austria. Book of Abstract page 48.
          2015
          Sarsu, F., Ashraf M., Caridad M., Kusolwa, P., Kihupi L., Rajab M., and N. Yona (2015) Rapid Screening Methods for Heat Stress Tolerance of Mutant Rice in the face of Climate Change.
          Jawaharlal Nehru University (JNU)- INDIA
          Plant Signaling/Journal
          2013
          Sumita Kumari, Suchismita Roy, Prabhjeet Singh, Sneh L. Singla-Pareek and Ashwani Pareek (2013). Cyclophilins: Proteins in search of function.
          Jawaharlal Nehru University (JNU)-INDIA
          Wageningen academic publisher. The Netherlands. ISBN: 978-90-8686-234-4. (in press)
          2013
          Priyanka Das, Manjari Mishra, Nita Lakra, Sneh L. Singla-Pareek (2013). Mutation Breeding: A Powerful Approach for Obtaining Abiotic Stress Tolerant Crops and Upgrading Food Security for Human Nutrition. In: Mutagenesis: exploring novel genes and pathways. Eds: Nasya Tomlekova, Imran Kozgar and Rafiq Wani.
          Zhejiang University- China
          Oral Presentation - International Conference on Genomics of Plant Genetic Resources:Chemically and Physically Induced Mutants:, 16-19 April 2013Jeju, Repulic of Korea
          2013
          Zhang H-L, Huang J-Z, Shu Q-Q, et al. An Overview of Molecular Nature and Prospect in Germplasm Innovation
          Centro Internacional de Agricultura Tropical (CIAT)- COLOMBIA
          Field Crops Research 148: 24-33.
          2013
          Assefa, T., S. E. Beebe, I. M. Rao, J. B. Cuasquer, M. C. Duque, M. Rivera, A. Battisti, M. Lucchin. 2013. Pod harvest index as a selection criterion to improve drought resistance in white pea bean.
          Zhejiang University
          Oral Presentation 16-19 April 2013, Jeju, Repulic of Korea 3rd International Conference on Genomics of Plant Genetic Resources Chemically and Physically Induced Mutants:
          2013
          Zhang H-L, Huang J-Z, Shu Q-Q, et al. An Overview of Molecular Nature and Prospect in Germplasm Innovation,
          China
          Hubei Agricultural Sciences, 2016, Vol. 55(23):6061-6066
          2016
          Identification of Heat Resistance in Radiation Breed Hybrid Rice ? YOU 838, Wang Y, Gao P, Fu Y, He J, Sun Q, Huang M
          China
          Plant Science Journal,2015,33(3):213-219
          2015
          Effects of High Temperature on the Activity and Expression of Antioxidative Enzymes in Rice Flag Leaves during Flowering Stage, Wang Y, Gao P, Huang M, Chen H, Yang ZR, Sun Qun. .
          China
          Paper, Rice. 7: 13
          2014
          Workable male sterility systems for hybrid rice: Genetics, biochemistry, molecular biology, and utilization. Huang J-Z, E Z-G, Zhang H-L, Shu Q-Y
          Netharland
          Book chapter -Academic Publishers, Pp 15-37.Editors: NasyaTomlekova, Imran Kozgar and Rafiq Wani. Publisher: Wageningen
          2013
          Mutation Breeding: A Powerful Approach for Obtaining Abiotic Stress Tolerant Crops and Upgrading Food Security for Human Nutrition, Priyanka Das, Manjari Mishra, Nita Lakra, Sneh Lata Singla-Pareek, Ashwani Pareek (2013).
          Shanghai, China
          The development of International isotopes and Radiation processing Seminar
          2011
          Detection of the antioxidase genes Expressionin in Radiation Breeding rice? YOU 838 after Heat Treatment. Wang Y, Gao P, Huang M, Wu L, Du XY, Lei
          Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
          BMC Genomics,Journal
          2012
          Pablo Peláez, Minerva S Trejo, Luis P I?iguez, Georgina Estrada-Navarrete, Alejandra A Covarrubias, José L Reyes and Federico Sanchez; Identification and characterization of microRNAs in Phaseolus vulgaris by high-throughput sequencing
          Centro Internacional de Agricultura Tropical (CIAT)-COLOMBIA
          In: Handbook of Plant and Crop Physiology. pp. 847-889. Pessarakli M., Eds. CRC Press, Taylor and Francis Group, USA.
          2014
          Rao, I. M. 2014. Advances in improving adaptation of common bean and Brachiaria forage grasses to abiotic stress in the tropics.
          Centro Internacional de Agricultura Tropical (CIAT)- COLOMBIA
          Plant Soil 372: 3-25.
          2013
          Yang, Z., I. M. Rao and W. J. Horst. 2013. Interaction of aluminium and drought stress on root growth and crop yield on acid soils.
          Centro Internacional de Agricultura Tropical (CIAT)- COLOMBIA
          Frontiers in Plant Physiology 4, 35 (1-20).
          2013
          Beebe, S., I. Rao, M. Blair and J. Acosta. 2013. Phenotyping common beans for adaptation to drought.
          Centro Internacional de Agricultura Tropical (CIAT)-COLOMBIA
          African Crop Science Journal 21: 265-281
          2013
          Rao, I., S. Beebe, J. Polania, J. Ricaurte, C. Cajiao, R. García and M. Rivera. 2013. Can tepary bean be a model for improvement of drought resistance in common bean? .
          Repulic of Korea
          Third International Conference on Genomics of Plant Genetic Resources, April 16-19, 2013, Jeju,
          Chemically and physically induced mutants: An overview of molecular nature and prospect in germplasm innovation. Shu Q-Y.
          Jawaharlal Nehru University (JNU)- INDIA
          Oral Presentation - International symposium on rice functional genomics
          2013
          Das P, Singla---Pareek SL and Pareek A; Characterization of gamma-ray induced rice mutant reveals the alteration in plant morphology, chloroplast structure, thylakoid organization and expression of transcripts involved in chlorophyll biosynthesis. International symposium on rice functional genomics. Page 101
          Jawaharlal Nehru University (JNU)-INDIA
          Oral Presentation-International symposium on rice functional genomics. Page 66.
          2013
          Roy S, Kumari S, Singh P, Singla---Pareek SL, Pareek A (2013). A highly abundant, stress regulated rice cyclophilin (OsCyp2) confers multistress tolerance to evolutionary diverse organisms.

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