Improving Nutritional Quality by Altering Concentrations of Enhancing Factors Using Induced Mutation and Biotechnology in Crops
Closed for Proposals
Project Type
Coordinated Research ProjectProject Code
D23028
CRP
1487
Approved Date
31 March 2008Project Status
ClosedStart Date
12 January 2009Expected End Date
30 April 2014Completed Date
28 November 2014Description
In addition to providing the major calories in human diets required to sustain life, plants are also the major source of health-beneficial agents, such as vitamins and minerals required for vital physiological processes. The major staple crops however, do not provide the essential vitamins and minerals in adequate quantities and quality. A sustainable way to ensure that adequate amounts of vitamins and minerals can be obtained from diets is to develop crops that provide the required amounts in their edible tissues. Induced mutations are a proven tool in creating desirable genetic variability in plants that translate to enhanced accumulation of essential minerals; synthesis of precursors of vitamins; modified quantities and qualities of starch, proteins and oils as well as secondary plant metabolites that play critical roles in improving human health and nutrition. This CRP will utilise efficient phenotypic screens and genotypic markers to trawl through mutant collections of selected model crops to identify putative mutants in traits of interest for incorporation into breeding programmes. Both the resulting genetic resources and the methodologies for identifying them constitute the main expected outputs from this proposed CRP. It is expected that plant scientists will adopt the putative mutants and hence facilitate the processes for breeding for improved nutritional quality in staple crops. Additionally, the screening methodologies (phenotypic and molecular) that will be validated with model crops will be adapted to suit other crops and quality constraints.
Objectives
To improve varieties of local crops with increased yield and crop quality, enhanced levels of micronutrient contents, other nutritional factors and market-preferred traits through induced mutation techniques and supportive biotechnologies.
Specific Objectives
To understand and identify genes involved in the biosynthesis of nutritional quality enhancing factors, and to develop efficient screening methods to facilitate the genetic improvement of nutritional quality. To transfer knowledge and technologies of beneficial mutants associated with nutritional factors from model crops to improve nutritional quality and yield into crops of socio economic importance to participating member states, specifically pepper, soybean groundnut, sweet potato, sorghum, wheat and maize.
Impact
? Development of hands-on protocols for screening and analysis of mutant populations
? Development of new germplasm collections through mutation assisted breeding in barley, sorghum, sweet potato, maize, wheat, tomato, potato, pepper, rice, soybean
? Release of one rice mutant variety and two maize mutant inbred lines with improved nutritional quality
? Development and commercialization of products from rice, wheat and barley mutant lines with health benefits
? Facilitated access to advanced analytical techniques
? Improvement of mutation rate in tomato through application of re-mutagenesis
? Increased knowledge of the metabolic pathways and genes affecting phytate, carotenoids, tocopherol, flavonoids and resistant starch biosynthesis
? Increased knowledge of methods to improve nutritional quality of model and targeted crops
? Dissemination of research results to the academic community through lectures to students, presentations at workshops and conferences and 40 scientific publications (papers and book chapters)
? Development of national and international collaboration
? Dissemination of information from this CRP through outreach activities including farmers days, academic and public lectures, community workshops, public exhibitions, policy and media briefings
? Sharing of knowledge and expertise within the CRP for mutual benefit
? Production of 8 PhD theses, 19 MSc theses, 8 BSc theses, on research directly contributing to this CRP
? Two CSIs trained in advanced techniques in agreement holders laboratories
? Access to other funding sources through successes of this CRP
? Enabled inter-disciplinary collaborations between crop scientists and nutritionists/health professionals
Relevance
Improving the delivery and bioavailability of essential vitamins and minerals to address nutritional deficiencies in an increasing number of people worldwide
CRP Publications
Bulgaria
Book
2014
Mutagenesis: Exploring Novel Genes and Pathways. (2014) Tomlekova N.B., Kozgar M.I., Wani M.R. (Eds), hardback, edited volume ISBN13: 978-90-8686-234-4; ISBN10: 9086862349, Publication Date: 2/6/2014, Publisher(s): Wageningen Academic Publishers, The Netherlands, Enfield Pub & Distribution Co, ± 500 pages.
Bulgaria
Book Chapter
2012
Tomlekova, N., Yancheva, S., Balacheva, E. and Atanasova, B. (2012) Molecular identification of tomato mutant lines. In: Kozgar, M.I. and Khan, S. (eds.) Induced mutations in crops. Bioremediation, Biodiversity and Bioavailability 6 ((Special Issue)), Global Science Books, Ltd., UK, (Special Issue 1): 58-64 (Print ISSN 1749-0596), book link:
Bulgaria
Book Chapter
2014
Tomlekova, N., Panayotov, N., Kozgar, M.I., Wani, M.R., Serdaris, P., Balacheva, E. (2014) Inducing and exploring new mutant donors of tomato. In: Tomlekova N.B., Kozgar M.I., Wani M.R. (Eds), Mutagenesis: Exploring Genetic Diversity in Crops, Wageningen Academic Publishers, The Netherlands, 285-308.
South Africa
Conference Proceedings
2012
N Chiloane, N Myeza, S Laurie, M Malebana. Application of in vitro techniques in mutation breeding of sweetpotato. 30th SA Society of Agric Technologists Congress, Salt Rock Hotel, Durban, 18-21 Sep 2012.
Bulgaria
Book Chapter
2012
Tomlekova, N. (2012) Genetic diversity of Bulgarian Phaseolus vulgaris L. germplasm collection through phaseolin and isozyme markers. In: ?aliskan M. (Ed), The Molecular Basis of Plant Genetic Diversity, http://www.intechopen.com/books/the-molecular-basis-of-plant-genetic-diversity, pp. 374, ISBN 978-953-51-0157-4, Rijeka: InTech. pp. 181-230
South Africa
Conference Proceedings
2009
LAURIE, S.M., et al., Initiation and first results of a Biofortification program for sweet potato in South Africa. J. Crop Improvement 23 (2009) 235.
Bulgaria
Scientific publication in peer reviewed journal
2013
Petrov, V., Denev I., Draganov M., Timin O., Panchev I., Tomlekova N. (2013) Molecular characterization of advanced mutants for early detection of high beta-carotene concentrations in pepper breeding programmes. Comptes rendus de l'Académie bulgare des sciences, 66(2), 303- 310
UK
Scientific publication in peer reviewed journal
2009
WHITE, P.J., et al., Relationships between yield and mineral concentrations in potato tubers, HortScience 44 (2009) 6-11
Bulgaria
Scientific publication in peer reviewed journal
2014
Petkova, V., Todorova V., Tomlekova N. (2014) Efficiency of the photosynthetic apparatus of sweet pepper (Capsicum annuum L.) F1 hybrids under high temperature stress, Plant Sciences (Bulgaria) 51: 43-48
South Africa (CSIR)
Scientific publication in peer reviewed journal
2011
da Silva, L.S., Jung, R., Zhao, Z.-y., Glassman, K., Grootboom, A.W., Mehlo, L., O'Kennedy, M.M., Taylor, J., Taylor, J.R.N. 2011. Effect of suppressing the synthesis of different kfirin subslasses on grain endosperm texture, protein body structure and protein nutritional quality in improved sorghum lines. Journal of Cereal Science 54 (1): 160-167.
South Africa (CSIR)
Scientific publication in peer reviewed journal
2013
Luke Mehlo,*, Zodwa Mbambo, Souleymane Bado, Johnson Lin, Sydwell M. Moagia,Sindisiwe Buthelezia, Stoyan Stoycheva, Rachel Chikwamba (2013) Induced protein polymorphisms and nutritional quality, Mutation Research, Elsevier B.V.,
China/Denmark
Scientific publication in peer reviewed journal
2013
Shu X, Jianwei Xu, Ying Wang, S?ren K. Rasmussen, Dianxing Wu. Effects of gamma irradiation on starch digestibility of rice with different resistant starch content. (2013) International Journal of Food Science & Technology, 48, 35-43
Bulgaria
Scientific publication in peer reviewed journal
2010
Nikolova, V., Todorova V., Stefanova Y., Tomlekova N. (2010) Cytological particularities in nuclear and nuclear-cytoplasmic male sterile pepper lines, Caryologia, 63:3, 262-268.
UK
Scientific publication in peer reviewed journal
2012
WHITE, P.J., BROADLEY, M.R., GREGORY, P.J., Managing the nutrition of plants and people, Appl. Environ. Soil Sci. 2012 (2012) article 104826
Peru
Conference Proceedings
2009
Gómez-Pando, L.; Eguiluz, A.; Jimenez, J.; Falconí, J; Heros E. 2009. Barley (Hordeunvulgare) and Kiwicha (Amaranthuscaudatus) Improvement by Mutation Induction in Peru. Q.Y. Shu (ed.), Induced Plant Mutations in the Genomics Era. Food and Agriculture Organization of the United Nations, Rome. 371-374.
UK
Scientific publication in peer reviewed journal
2012
WHITE, P.J., et al., Biofortification of potato tubers using foliar zinc-fertilisers, J. Hort. Sci. Biotechnol. 87 (2012) 123-129.
Bulgaria
Book Chapter
2014
Tomlekova, N., Timin, O., Arnaoudova, Y., Timina, O., Wani, M.R., Kozgar, M.I. (2014) Trends and achievements in F1 hybrids of sweet pepper utilizing induced male-sterility In: Tomlekova N.B., Kozgar M.I., Wani M.R. (Eds), Mutagenesis: Exploring Genetic Diversity in Crops, Wageningen Academic Publishers, The Netherlands, 13-38.
South Africa
Conference Proceedings
2009
LAURIE, S.M., et al., Quick screening method for drought tolerance for sweet potato tested in South Africa. Triennial Symposium of International Society of Tropical and Subtropical Crops (ISTRC), 2-6 Nov 2009, CIP-HO/UANALM, Lima, Peru (2009).
China/Denmark
Scientific publication in peer reviewed journal
2014
Shu X, Rasmussen SK. Quantification of amylose, amylopectin, and ?-glucan in search for genes controlling the three major quality traits in barley by genome-wide association studies. (2014) Front Plant Sci. 15;5:197. doi: 10.3389/fpls.2014.00197.
Bulgaria
Conference Proceedings
2012
Migdalis, N., Serdaris P., Tomlekova N. (2012) Desert Challenge for Western Brands: the United Arab Emirates Experience. 1. The emirates as a place of internal commerce and behavior of one of the components of the market – the users. Proceedings of the International Conference on Management and Entrepreneurship, Technical University, 19 - 20 October 2012, Plovdiv, 164-169.
Germany
Scientific publication in peer reviewed journal
2011
Wenke T, D?bel T, S?rensen TR, Junghans H, Weisshaar B, Schmidt T (2011) Targeted identification of short interspersed nuclear element families shows their widespread existence and extreme heterogeneity in plant genomes. Plant Cell 23(9):3117-28
Bulgaria
Book Chapter
2014
Tomlekova, N. (2014) Use of mutant genes for exploring and altering carotenoid biosynthetic pathway of sweet pepper: The Bulgarian viewpoint. In: Tomlekova N.B., Kozgar M.I., Wani M.R. (Eds), Mutagenesis: Exploring Novel Genes and Pathways, Wageningen Academic Publishers, The Netherlands, 433-454.
Denmark
Scientific publication in peer reviewed journal
2013
Mark AB, Kápolna E, Laursen KH, Halekoh U, Rasmussen SK, Husted S, Larsen EH, Bügel S. Consumption of organic diets does not affect intake and absorption of zinc and copper in men - evidence from two cross-over trials. (2013) Food Funct. 4(3):409-19.
Germany
Scientific publication in peer reviewed journal
2012
Seibt KM, Wenke T, Wollrab C, Junghans H, Muders K, Dehmer KJ, Diekmann K, Schmidt T (2012) Development and application of SINE-based markers for genotyping of potato varieties. Theor Appl Genet 125(1):185-96
Bulgaria
Scientific publication in peer reviewed journal
2011
Timina, O.O., Timin O.Y., Fiodoroff S.K., Tomlekova N. (2011) Inheritance of pericarp color pattern and ?-carotene content in vegetable pepper. Vavilov Journal of Genetics and Breeding, /In Russian/, 15(3): 585-594.
Bulgaria
Conference Proceedings
2012
Migdalis, N., Serdaris P., Tomlekova N. (2012) Desert Challenge for Western Brands: the United Arab Emirates Experience. 2. Possibilities of the European brands to sell on the emirates’ markets. Proceedings of the International Conference on Management and Entrepreneurship, Technical University, 19 - 20 October 2012, Plovdiv, 170-175.
Bulgaria
Scientific publication in peer reviewed journal
2012
Balacheva, E., Kartzeva T., Atanassova B., Tomlekova N. (2012) The Diversity in Tomato Colours – Potential for Diversity in Tomato Nutritive Quality. Acta Horiculturae, 960, 31-36.
UK
Scientific publication in peer reviewed journal
2009
WHITE, P.J., BROADLEY, M.R., Biofortification of crops with seven mineral elements often lacking in human diets - iron, zinc, copper, calcium, magnesium, selenium and iodine, New Phytol. 182 (2009) 49-84.
China
Scientific publication in peer reviewed journal
2009
Shu X, Limeng Jia, Hongxia Ye, Chengdao Li, Dianxing Wu. Slow digestion properties of rice differential in resistant starch. Journal of Agricultural and Food Chemitry. 2009, 57: 7552-7559
Bulgaria
Conference Proceedings
2014
Spasova-Apostolova, V.V., Tomlekova, N.B., Todorova, V.Y., 2014. Conducting marker-assisted selection in pepper for increasing quality and selection of donors for F1 hybrids. 30-31.10.2013, Scientific works. Union of Scientists in Bulgaria - Plovdiv. Series “Techniques and technologies”. Proceeding of the conference of Union of the Scientists of Bulgaria – Plovdiv, pp. 21-26.
Denmark
Scientific publication in peer reviewed journal
2013
Roos N, S?rensen JC, S?rensen H, Rasmussen SK, Briend A, Yang Z, Huffman SL. Screening for anti-nutritional compounds in complementary foods and food aid products for infants and young children. Matern Child Nutr. 2013 Jan;9 Suppl 1:47-71.
Bulgaria
Book
2014
Mutagenesis: Exploring Genetic Diversity in Crops. (2014) Tomlekova N.B., Kozgar M.I., Wani M.R. (Eds), Edited by Nasya Tomlekova, Imran Kozgar and Rafiq Wani (Editors), hardback, edited volume ISBN13:; ISBN10: 9086862349, Publication Date: 15/6/2014, Publisher(s): Wageningen Academic Punblishers, The Netherlands, Enfield Pub & Distribution Co, ± 400 pages
Bulgaria
Book
2013
Poryazov, I.B., Petkova, V.P., Tomlekova, N.B. (2013) Vegetable production, ‘DIMI 99’ Ltd., Sofia, ISBN: 978-954-765-064-0, 318 p.
China/Denmark
Scientific publication in peer reviewed journal
2012
Shu X, Gunter Backes, S?ren K.Rasmussen. Genome-wide association study of resistant starch (RS) phenotypes in a barley variety collection. Journal of Agricultural and Food Chemistry. 2012, 60: 10302-10322
Bulgaria
Scientific publication in peer reviewed journal
2010
Tomlekova, N.B. (2010) Induced mutagenesis for crop improvement in Bulgaria. Plant Mutation Report, 2(2): 1-32.
South Africa
Conference Proceedings
2012
M.E. Malebana, S.M. Laurie, N. Chiloane, N. Myeza & R. Kleynhans. Radio-sensitivity results towards induced mutations for improved nutritional quality and drought tolerance in sweet potato (Ipomoea batatas L. LAM). SA Plant Breeders Symposium, 11-14 Mar 2012, Skukuza.
UK
Scientific publication in peer reviewed journal
2011
WHITE, P.J., BROADLEY, M.R., Physiological limits to zinc biofortification of edible crops, Frontiers Plant Sci. (2011) 2:80.
Bulgaria
Scientific publication in peer reviewed journal
2012
Nacheva, E., Tomlekova, N., Petkova, V., 2012. Induced mutagenesis in F1 hybrid seeds of potato. Journal of Ecology and Future, vol. XI, ? 4, 30-35.
Denmark
Scientific publication in peer reviewed journal
2010
Rasmussen SK, Ingvardsen CR, Torp AM. Mutations in genes controlling the biosynthesis and accumulation of inositol phosphates in seeds. (2010) Biochem Soc Trans. 38(2):689-94. (Review).
Bulgaria
Scientific publication in peer reviewed journal
2010
Draganov, M., Miloshev G., Popov N., Murdjeva M., Staneva D., Kostova M., Draganov D., Tomlekova N. (2010) Molecular and cytogenetics criteria for identification of serum-free cell cultures. Biotechnology & Biotechnological Equipment, 24: 139-143.