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          Radiation Inactivation of Bio-hazards Using High Powered Electron Beam Accelerators

          Closed for Proposals

          Project Type

          Coordinated Research Project

          Project Code

          F23033

          CRP

          2220

          Approved Date

          08/11/2017

          Project Status

          Closed

          Start Date

          13/04/2018

          Expected End Date

          12/04/2022

          Completed Date

          30/06/2025

          Participating Countries

          Argentina, Brazil, Canada, Germany, Egypt, Hungary, Republic of Korea, Kuwait, Malaysia, Poland, Portugal, Tunisia, Türkiye, United States of America

          Description

          Radiation technology has emerged as an effective tool for decontamination of biohazards. It has been instrumental in improving the quality of human life through its proven effectiveness in sterilization of medical and healthcare products, and also for improving food safety. Over the years this technology has been primarily based on using gamma radiation sources like Co-60 as the radiation source. However, the emerging concerns regarding the safety and security of radioactive sources coupled with issues related to shipping such materials through normal supply chain process have encouraged industry to look for machine based electron beam radiation sources in future. To bring out the full potential of electron beams in these areas related to treatment of biohazards, it is important to establish a number of factors that include the kinetics of inactivation which is complicated on account of the variety of radiation sources, their dose rates, and energies as well as because of differences in the nature of microbes and their environment. This?Coordinated Research Project (CRP) relates to establishing the kinetics of inactivation of microbes at high dose rates so as to use electron beam technology for inactivation of biohazards.

          Objectives

          Formulate guidelines to enhance and strengthen use of electron beam accelerators for treatment of biohazards of concern under changing conditions such as at high dose rates, different ambient conditions, and varying substrates in applications such as radiation sterilization, hygienization of bio-solids, sanitizing infectious hospital waste or toxic effluents and eliminating deliberate biohazards.

          Specific Objectives

          Demonstrate treatment of biohazards using electron accelerators.

          Develop validation procedures for the processes of treating biohazards at high dose rates

          Develop guidelines for treatment of biohazards using electron beam accelerators

          Dose setting procedures appropriate to specific bio contaminants.

          Provide reliable data about radiation inactivation of pathogenic microorganisms and other bio contaminants under varying physical conditions.

          Develop appropriate methodologies for treatment of biohazards using electron beam accelerators

          Impact

          The impact of the CRP F23033, aimed at enhancing and strengthening the use of electron beam (e-beam) accelerators for treating biohazards is multifaceted. First of all, it was clearly demonstrated that electron beam accelerators effectively kill bacteria, viruses, and other pathogens by damaging their DNA/RNA, preventing reproduction. This technology can be used to disinfect medical supplies, protective equipment, and even wastewater, providing a rapid method to control pathogens. By treating biohazardous materials (such as medical waste or contaminated animal products) with e-beams, the spread of infectious diseases, which is crucial in preventing pandemics, can be significantly reduced. Second, as e-beam sterilization is a clean process, it significantly reduces the need for harsh chemicals (e.g., ethylene oxide, formaldehyde) traditionally used in sterilization. This minimizes the release of toxic byproducts, which is beneficial for both the environment and human health. Accelerated biodegradation of organic waste, like animal or plant byproducts, can help manage waste efficiently. Treated bio-waste can even be reused safely, potentially as fertilizer or other byproducts, contributing to a circular economy model. In addition, electron beam technology proved to be useful for food sterilization as it reduces or eliminates pathogens without significant alteration to the food's properties or nutritional content. This enhances food safety, shelf life, and supports export quality standards. Finally, this project encouraged the development of scalable, cost-effective e-beam technology, supporting new applications and research, from industrial sterilization to experimental treatments for other contaminants (e.g., pharmaceutical residues).

          Relevance

          This CRP is relevant to several IAEA topics (see below) and other CRPs, for example F23034, F23035, and D61025

          CRP Publications

          Germany
          Journal Article
          2020
          Automated application of low energy electron irradiation enables inactivation of pathogenand cell-containing liquids in biomedical research and production facilities
          USA, TAMU
          Research article
          2019
          A research article on "A Comparative Analysis of the Metabolomic Response of Electron Beam Inactivated E. coli O26:H11 and Salmonella Typhimurium ATCC 13311"
          USA
          Journal Article
          2022
          Assessment of microbiological correlates and immunostimulatory potential of Electron Beam Inactivated Metabolically Active Yet Non Culturable (MAyNC) Salmonella Typhimurium
          Poland, INCT
          Report
          2019
          A report on "WYKORZYSTANIE PROMIENIOWANIA JONIZUJACEGO W OBRóBCE OSADóW Z OCZYSZCZALNI SCIEKóW. ?ZEROENERGETYCZNA” TECHNOLOGIA DO OBRóBKI OSADóW SCIEKOWYCH"
          Poland, INCT
          Research article
          2019
          A research article on "‘Zero energy’ electron beam technology for sludge hygienization"
          Brazil, IPEN
          Research article
          2018
          A research article on "Emerging pollutants, related toxicity, and water quality decreasing: tannery, textile, and pharmaceuticals load pollutants"
          Hungary
          Conference Paper
          2019
          Preventing the Development of Antibiotic Resistance in Wastewater Matrices by High Energy Irradiation
          Portugal
          Journal Article
          2020
          Ionizing Radiation Technologies to Increase the Extraction of Bioactive Compounds from Agro-Industrial Residues: A Review
          Brazil, IPEN
          Research article
          2021
          A research article on "Mycoflora and DNA sequencing analysis of radioresistant fungi isolated from irradiated vehicular air conditioning filters collected in S?o Paulo, Brazil"
          Poland
          Conference Paper
          2020
          NEW TRENDS IN ELECTRON BEAM ENVIRONMENTAL APPLICATIONS
          Canada
          Journal Article
          2020
          Microbial radiosensitization using combined treatments of essential oils and irradiation- part B: Comparison between gamma-ray and X-ray at different dose rates
          Poland, INCT
          Conference presentation
          2020
          A conference presentation on "A METHOD OF HYGIENISATION AND DISINTEGRATION"
          Canada, Canadian Irradiation Centre
          Research article
          2020
          A research article on "Radiosensitivity of Feline Calicivirus F9 on Iceberg Lettuce Surface after Combined Treatments with ?-Radiation"
          Canada, Canadian Irradiation Centre
          Research article
          2021
          A research article on "Radiosensitization of rice weevil Sitophilus oryzae using combined treatments of essential oils and ionizing radiation with gamma-ray and X-Ray at different dose rates"
          USA, TAMU
          Research article
          2020
          A research article on "A critical review of ionizing radiation technologies for the remediation of waters containing Microcystin-LR and M. aeruginosa"
          Canada
          Journal Article
          2020
          Radiosensitivity increase in FCV-F9 virus using combined treatments with natural antimicrobials and gamma-irradiation
          Portugal
          Journal Article
          2020
          Effect of Gamma-Radiation on Zearalenone— Degradation, Cytotoxicity and Estrogenicity
          Germany, Fraunhofer Institute for Organic Electronics
          Research article
          2018
          A research article on "Low-energy electron-beam treatment as alternative for on-site sterilization of highly functionalized medical products – A feasibility study"
          Canada
          Journal Article
          2020
          Potential synergistic antimicrobial efficiency of binary combinations of essential oils against Bacillus cereus and Paenibacillus amylolyticus-Part A
          Poland, INCT
          Conference presentation
          2020
          A conference presentation on "NEW TRENDS IN ELECTRON BEAM"
          Canada
          Journal Article
          2020
          Effect of gamma-irradiation on the microbial inactivation, nutritional value, and antioxidant activities of infant formula
          Germany, Fraunhofer Institute for Organic Electronics
          Research article
          2019
          A research article on "Eimeria tenella oocysts attenuated by low energy electron irradiation (LEEI) induce protection against challenge infection in chickens"
          Portugal, C2TN
          Research article
          2020
          A research article on "Ionizing Radiation Technologies to Increase the Extraction of Bioactive Compounds from Agro-Industrial Residues: A Review"
          Canada
          Journal Article
          2020
          Radiosensitivity of Feline Calicivirus F9 on Iceberg Lettuce Surface after Combined Treatments with c-Radiation
          USA
          Journal Article
          2021
          Controlling the Colonization of Clostridium perfringens in Broiler Chickens by an Electron-Beam-Killed Vaccine
          USA, TAMU
          Research Article
          2021
          A research article on "Controlling the Colonization of Clostridium perfringens in Broiler Chickens by an Electron-Beam-Killed Vaccine"
          Germany, Fraunhofer Institute for Organic Electronics
          Research article
          2020
          A research article on "Low-Energy Electron Irradiation Efficiently Inactivates the Gram-Negative Pathogen Rodentibacter pneumotropicus-A New Method for the Generation of Bacterial Vaccines with Increased Efficacy"
          USA
          Journal Article
          2020
          Benchmarking the minimum Electron Beam (eBeam) dose required for the sterilization of space foods
          Canada, Canadian Irradiation Centre
          Research article
          2020
          A research article on "Microbicidal effectiveness of irradiation from Gamma and X-ray sources at different dose rates against the foodborne illness pathogens Escherichia coli, Salmonella Typhimurium and Listeria monocytogenes in rice"
          Portugal, C2TN
          Research article
          2020
          A research article on "The use of gamma radiation for extractability improvement of bioactive compounds in olive oil wastes"
          Argentina
          Conference Paper
          2019
          Inactivación microbiana en residuos de animales de laboratorio utilizando radiaciones ionizantes
          Canada
          Journal article
          2020
          Microbicidal effectiveness of irradiation from Gamma and X-ray sources at different dose rates against the foodborne illness pathogens Escherichia coli, Salmonella Typhimurium and Listeria monocytogenes in rice
          Poland
          Journal Article
          2019
          https://iaeacloud.sharepoint.com/:b:/r/sites/CRADocuments/CRPs/F23033%202220/Publications/POL1.pdf?csf=1&web=1&e=MoRZS4
          USA, TAMU
          Research article
          2018
          A research article on "Escherichia coli Cells Exposed to Lethal Doses of Electron Beam Irradiation Retain Their Ability to Propagate Bacteriophages and Are Metabolically Active"
          USA, TAMU
          Research article
          2020
          A research article on "Reduction in microbial infection risks from raw milk by Electron Beam Technology"
          Hungary
          Journal Article
          2021
          Antibiotics in a wastewater matrix at environmentally relevant concentrations affect coexisting resistant/sensitive bacterial cultures with profound impact on advanced oxidation treatment
          Brazil
          Journal Article
          2018
          Emerging pollutants, related toxicity, and water quality decreasing: Tannery, textile, and pharmaceuticals load pollutants
          USA
          Journal Article
          2018
          Escherichia coli Cells Exposed to Lethal Doses of Electron Beam Irradiation Retain Their Ability to Propagate Bacteriophages and Are Metabolically Active
          USA
          Journal Article
          2020
          A critical review of ionizing radiation technologies for the remediation of waters containing Microcystin-LR and M. aeruginosa
          Poland
          Journal Article
          2019
          WYKORZYSTANIE PROMIENIOWANIA JONIZUJACEGO W OBRóBCE OSADóW Z OCZYSZCZALNI SCIEKóW. ?ZEROENERGETYCZNA” TECHNOLOGIA DO OBRóBKI OSADóW SCIEKOWYCH
          Canada, Canadian Irradiation Centre
          Research article
          2020
          A research article on "Potential synergistic antimicrobial efficiency of binary combinations of essential oils against Bacillus cereus and Paenibacillus amylolyticus-Part A"
          Brazil
          Journal Article
          2021
          Mycoflora and DNA sequencing analysis of radioresistant fungi isolated from irradiated vehicular air conditioning filters collected in S?o Paulo, Brazil
          Canada, Canadian Irradiation Centre
          Research article
          2020
          A research article on "Effect of ?-irradiation on the microbial inactivation, nutritional value, and antioxidant activities of infant formula"
          USA
          Journal Article
          2020
          Reduction in microbial infection risks from raw milk by Electron Beam Technology
          Germany
          Journal Article
          2019
          Eimeria tenella oocysts attenuated by low energy electron irradiation (LEEI) induce protection against challenge infection in chickens
          Argentina
          Journal article
          2021
          MICROBIAL INACTIVATION IN ANIMAL WASTE WITH IONIZING RADIATION
          Germany
          Journal Article
          2018
          Low-energy electron-beam treatment as alternative for on-site sterilization of highly functionalized medical products – A feasibility study
          USA
          Journal Article
          2019
          A Comparative Analysis of the Metabolomic Response of Electron Beam Inactivated E. coli O26:H11 and Salmonella Typhimurium ATCC 13311
          Canada
          Journal Article
          2021
          Radiosensitization of rice weevil Sitophilus oryzae using combined treatments of essential oils and ionizing radiation with gamma-ray and X-Ray at different dose rates
          USA, TAMU
          Research Article
          2018
          A research article on "Benchmarking the minimum Electron Beam (eBeam) dose required for the sterilization of space foods"
          Hungary, Institute for Energy Security and Environmental Safety
          Research article
          2021
          A research article on "Antibiotics in a wastewater matrix at environmentally relevant concentrations affect coexisting resistant/sensitive bacterial cultures with profound impact on advanced oxidation treatment"
          Portugal, C2TN
          Research article
          2020
          A research article on "Effect of Gamma-Radiation on Zearalenone—Degradation, Cytotoxicity and Estrogenicity"
          Canada, Canadian Irradiation Centre
          Research article
          2020
          A research article on "Radiosensitivity increase in FCV-F9 virus using combined treatments with natural antimicrobials and gamma-irradiation"
          Germany, Fraunhofer Institute for Organic Electronics
          Research article
          2020
          A research article on "Automated application of low energy electron irradiation enables inactivation of pathogen- and cell-containing liquids in biomedical research and production facilities"
          Germany
          Journal Article
          2020
          Low-Energy Electron Irradiation Efficiently Inactivates the Gram-Negative Pathogen Rodentibacter pneumotropicus—A New Method for the Generation of Bacterial Vaccines with Increased Efficacy
          Portugal
          Journal Article
          2020
          The use of gamma radiation for extractability improvement of bioactive compounds in olive oil wastes
          Canada, Canadian Irradiation Centre
          Research article
          2020
          A research article on "Microbial radiosensitization using combined treatments of essential oils and irradiation- part B: Comparison between gamma-ray and X-ray at different dose rates"
          Argentina, CNEA
          Book chapter
          2021
          Book chapter "MICROBIAL INACTIVATION IN ANIMAL WASTE WITH IONIZING RADIATION" in a book "MICROBIOLOGIA: CLINICA, AMBIENTAL E ALIMENTOS"
          Argentina, CNEA
          Conference presentation
          2019
          Presentationa at the IV Congreso Nacional de Ciencia y Tecnología Ambiental "Inactivación microbiana en residuos de animales de laboratorio utilizando radiaciones ionizantes"

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