Integration of the SIT with Biocontrol for Greenhouse Insect Pest Management
Closed for Proposals
Project Type
Coordinated Research ProjectProject Code
D43003
CRP
2116
Approved Date
2016/09/14Project Status
ClosedStart Date
2017/03/17Expected End Date
2023/03/16Completed Date
2023/07/12Participating Countries
Argentina, Brazil, Canada, Chile, Egypt, France, Guatemala, India, Iran, Islamic Republic of, Italy, Mexico, New Zealand, PakistanDescription
This CRP will develop and evaluate SIT for insect pests in greenhouses. The CRP will focus on three groups of species that are disruptive to current biocontrol practices, or are expected to become serious pest when invading new areas. The three groups are Drosophila suzukii, Heilcoverpa / Spodoptera spp. and Tuta absoluta / Neoleucinodes elegantalis. The background information required for SIT, such as mass-rearing and radiation biology, has been developed for these greenhouse pests to a variable extent. However, for all of these groups the application in confined areas still needs to be refined and evaluated.
Objectives
The overall objective is to advance development and implementation of SIT for integration with other biocontrol in greenhouses.
Specific Objectives
To develop SIT and inherited sterility for Tuta absoluta and Neoleucinodes elegantalis
To adapt SIT and inherited sterility for Spodoptera/Helicoverpa species for confined cropping systems
To develop SIT for Drosophila suzukii
To develop SIT and inherited sterility for Tuta absoluta and Neoleucinodes elegantalis
To adapt SIT and inherited sterility for Spodoptera/Helicoverpa species for confined cropping systems
To develop SIT for Drosophila suzukii
Impact
1. Guidelines for D suzukii mass rearing and irradiation protocols http://www.dgdingfa.net/sites/default/files/massrearing-and-irradiation-sw… provides tools and knowledge for member states.
2. Quality control parameters guidelines under development will provide knowledge and tools for member states.
3. Knowing the effect of biological control agents gives new residue-free options for D. suzukii suppression.
4. Population models and molecular markers provide guidance on numbers of sterile insects and BCAs for D. suzukii management.
5. Desirable phenotypic traits to improve mating success of mass-reared sterilized insects were identified.
6. Dose response of parental, and subsequent sterilization of F1, has been established for Tuta absoluta, Helicoverpa armigera and Spodoptera littoralis.
7. Diets have been developed for T. absoluta, H. armigera and S. littoralis mass rearing.
8. No asexually reproducing populations of T. absoluta have been detected in Argentina meaning that SIT remains viable for Argentinian populations.
9. The presence of Wolbachia in T. absoluta from India (Supergroup A and B) and Argentina (Supergroup B) means that cytoplasmic incompatibility is a possible tool for T. absoluta management.
10. Biocontrol agents like Chrysoperla sp., Tupiocoris sp. Trichogramma sp., have been validated as viable control agents for T. absoluta and S. littoralis.
The results achieved during this CRP have now made it possible to manage Drosophila suzukii and Tuta absoluta with the sterile insect technique and with biological control agents. Key diet development and dose response steps have been achieved to realize the implementation of the sterile insect technique and inherited sterility technique for future Helicoverpa armigera and Spodoptera littoralis management.
Relevance
The CRP pioneered the development of the sterile insect technique for Drosophila suzukii and Tuta absoluta. The results of the research undertaken during the CRP have provided novel information regarding the dose response, mass rearing diet and insect collection and the use of biological control agents for population suppression of Drosophila suzukii and Tuta absoluta. Furthermore, the methodologies have been transferred to Helicoverpa armigera and Spodoptera littoralis. Specific mass rearing and irradiation recommendations have been provided in an IAEA guideline document. Semi-field trials have validated the SIT and BCA strategies as viable options for D. suzukii and T. absoluta. Wolbachia was identified from Tuta populations, paving the way for the development of management tools utilizing cytoplasmic incompatibility.
CRP Publications
Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Reserch article
2019
Mass-Rearing of Drosophila suzukii for Sterile Insect Technique Application: Evaluation of Two Oviposition Systems
Brazil, Departamento de Fitossanidade, Universidade Federal de Pelotas, Pelotas, RS, Brazil. Type: Research article
Research article
2019
Radiation effects on Drosophila suzukii (Diptera: Drosophilidae) reproductive behaviour
Argentina - Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina.
Research article
2021
The pest kill rate of thirteen natural enemies as aggregate evaluation criterion of their biological control potential of Tuta absoluta. Scientific Reports. 11, 10756.
Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Research article
2021
Hypoxia combined with chilling maintains the quality of irradiated Drosophila flies: a simulated shipment experiment
Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Reserch article
2019
Mass-Rearing of Drosophila suzukii for Sterile Insect Technique Application: Evaluation of Two Oviposition Systems
Mexico / Programa Moscafrut SADER-SENASICA, Subdirección de Desarrollo de Métodos, Camino a Cacaotales s/n 30860, Metapa de Domínguez, Chiapas, México
Reserch article
2020
Mass Rearing, Quality Parameters, and Bioconversion in Drosophila suzukii (Diptera: Drosophilidae) for Sterile Insect Technique Purposes
Germany
Institute for Insect Biotechnology, Justus-Liebig-University Giessen, Giessen, Germany
2018
Environmentally sustainable pest control options for Drosophila suzukii
Germany Institute for Insect Biotechnology, Justus-Liebig-University Giessen, Giessen, Germany
Research article
2018
Environmentally sustainable pest control options for Drosophila suzukii
Mexico / Programa Moscafrut SADER-SENASICA, Subdirección de Desarrollo de Métodos, Camino a Cacaotales s/n 30860, Metapa de Domínguez, Chiapas, México
Reserch article
2020
Mass Rearing, Quality Parameters, and Bioconversion in Drosophila suzukii (Diptera: Drosophilidae) for Sterile Insect Technique Purposes
Mexico / Programa Moscafrut SADER-SENASICA, Subdirección de Desarrollo de Métodos, Camino a Cacaotales s/n 30860, Metapa de Domínguez, Chiapas, México
Reserch article
2020
Mass Rearing, Quality Parameters, and Bioconversion in Drosophila suzukii (Diptera: Drosophilidae) for Sterile Insect Technique Purposes
Germany
Institute for Insect Biotechnology, Justus-Liebig-University Giessen, Giessen, Germany
2018
Environmentally sustainable pest control options for Drosophila suzukii
Germany Institute for Insect Biotechnology, Justus-Liebig-University Giessen, Giessen, Germany
Reserch article
2018
Environmentally sustainable pest control options for Drosophila suzukii
Biological Application Department, Nuclear research center, Atomic Energy Authority, Cairo, Egypt and Faculty of Agriculture, Department of Economic Entomology and pesticide, Cairo university Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Research article
2018
Effect of gamma irradiation on the susceptibility of the cotton leaf worm, Spodoptera littoralis (Bosid.) (Lepidoptera: Noctuidae) to the infection with nucleopolyhedrosis virus
USA / Italy - Department of Horticulture, Oregon State University / Technology Transfer Centre, Fondazione Edmund Mach, Italy
Reserch article
2019
Augmentative releases of Trichopria drosophilae for the suppression of early season Drosophila suzukii populations
India- Nuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre
Research article
2018
Development of ecofriendly sterile insect technique for agriculturally important insect pests
Brazil, Departamento de Fitossanidade, Universidade Federal de Pelotas, Pelotas, RS, Brazil. Type: Research article
Research article
2018
Effects of irradiation dose on sterility induction and quality parameters of Drosophila suzukii (Diptera: Drosophilidae)
FAO/IAEA - IPCL
Guideline
2022
Guidelines for Mass Rearing and Irradiation of Drosophila suzukii for Sterile Insect Technique Application
Argentina - Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina.
_Research article
2021
Analysis of parthenogenesis and molecular detection of reproductive symbionts in Tuta absoluta (Lepidoptera: Gelechiidae) from Argentina. Entomologia Experimentalis et applicata.
Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Reserch article
2019
Mass-Rearing of Drosophila suzukii for Sterile Insect Technique Application: Evaluation of Two Oviposition Systems
Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Reserch article
2019
Canada / Austria - Institut de recherche et de de′veloppement en agroenvironnement, Saint-Bruno-de-Montarville, Que′bec, - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Research article
2017
The sterile insect technique for the management of the spotted wing drosophila, Drosophila suzukii: Establishing the optimum irradiation dose
India- Nuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre
Research article
2018
Molecular characterisation of tomato leaf miner Tuta absoluta populations obtained from different geographical locations of India.
USA / Italy - Department of Horticulture, Oregon State University / Technology Transfer Centre, Fondazione Edmund Mach, Italy
Reserch article
2019
Augmentative releases of Trichopria drosophilae for the suppression of early season Drosophila suzukii populations
Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Reserch article
2019
Italy, University of Pavia, Department of Biology and Biotechnology
Reserch article
2022
Polyandry is an important reproductive trait of the invasive Drosophila suzukii
Canada / Austria - Institut de recherche et de de′veloppement en agroenvironnement, Saint-Bruno-de-Montarville, Que′bec, - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Reserch article
2017
The sterile insect technique for the management of the spotted wing drosophila, Drosophila suzukii: Establishing the optimum irradiation dose
Austria - France / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture - Université de Rennes
Reserch article
2018
Sterile insect technique and Wolbachia symbiosis as potential tools for the control of the invasive species Drosophila suzukii
Université de Carthage, Institut Supérieur des études Préparatoires en BiologieGéologie (ISEP-BG) / Tunisia
Reserch article
2019
Occurrence, biology, natural enemies and management of Tuta absoluta in Africa
USA / Italy - Department of Horticulture, Oregon State University / Technology Transfer Centre, Fondazione Edmund Mach, Italy
Research article
2019
Augmentative releases of Trichopria drosophilae for the suppression of early season Drosophila suzukii populations
Italy, University of Pavia, Department of Biology and Biotechnology
Scientific presentation
2022
Unravelling the reproductive biology traits of Drosophila suzukii as a basis for specific and long-term control of this species
Biological Application Department, Nuclear research center, Atomic Energy Authority, Cairo, Egypt joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Research article
2020
Increasing the efficacy of the cotton leaf worm Spodoptera littoralis nucleopolyhedrosis virus using certain essential oils
Canada / Austria - Institut de recherche et de de′veloppement en agroenvironnement, Saint-Bruno-de-Montarville, Que′bec, - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Reserch article
2017
The sterile insect technique for the management of the spotted wing drosophila, Drosophila suzukii: Establishing the optimum irradiation dose
Canada / Austria - Institut de recherche et de de′veloppement en agroenvironnement, Saint-Bruno-de-Montarville, Que′bec, - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Reserch article
2017
The sterile insect technique for the management of the spotted wing drosophila, Drosophila suzukii: Establishing the optimum irradiation dose
México, SENASICA-CNRF, Carretera Federal México-Pachuca Km 37.5 C.P. 55740, Tecámac, Estado de México Departamento de Fitossanidade
Research article
2019
Effect of irradiation on quality and fertility parameters of Drosophila suzukii in Mexico
Université de Carthage, Institut Supérieur des études Préparatoires en Biologie Géologie (ISEP-BG) / Tunisia Year: 2019
Research article
2019
Occurrence, biology, natural enemies and management of Tuta absoluta in Africa
Austria, Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Scientific poster
2022
USA / Italy - Department of Horticulture, Oregon State University / Technology Transfer Centre, Fondazione Edmund Mach, Italy
Reserch article
2019
Augmentative releases of Trichopria drosophilae for the suppression of early season Drosophila suzukii populations
Université de Carthage, Institut Supérieur des études Préparatoires en BiologieGéologie (ISEP-BG) / Tunisia
2019
Occurrence, biology, natural enemies and management of Tuta absoluta in Africa
Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Research article
2019
Mass-Rearing of Drosophila suzukii for Sterile Insect Technique Application: Evaluation of Two Oviposition Systems
Biological Application Department, Nuclear research center, Atomic Energy Authority, Cairo, Egypt , Department of plant protection Faculty of Agriculture, Fayoum University joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Research article
2021
Effect of low cost diet on the cotton leaf worm , Spodoptera littoralis nucleopolyhedrosis virus pathogenicity and sterile insect technique
Université de Carthage, Institut Supérieur des études Préparatoires en BiologieGéologie (ISEP-BG) / Tunisia
2019
Occurrence, biology, natural enemies and management of Tuta absoluta in Africa
Organization: Mexico / Programa Moscafrut SADER-SENASICA, Subdirección de Desarrollo de Métodos, Camino a Cacaotales s/n 30860, Metapa de Domínguez, Chiapas, México
Research article
2021
Pathogenicity and virulence of Isaria javanica, Metarhizium anisopliae, and Beauveria bassiana strains for control of Drosophila suzukii (Matsumura)
Austria - France / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture - Université de Rennes
Reserch article
2019
Sterile insect technique and Wolbachia symbiosis as potential tools for the control of the invasive species Drosophila suzukii
Austria / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Reserch article
2019
Irradiation dose response under hypoxia for the application of the sterile insect technique in Drosophila suzukii
Austria - France / BOKU, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences - Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture - Université de Rennes
Reserch article
2018
Sterile insect technique and Wolbachia symbiosis as potential tools for the control of the invasive species Drosophila suzukii