Strengthening of “Biological dosimetry” in IAEA Member States: Improvement of current techniques and intensification of collaboration and networking among the different institutes
Closed for Proposals
Project Type
Coordinated Research ProjectProject Code
E35008
CRP
1833
Approved Date
17 September 2011Project Status
ClosedStart Date
10 February 2012Expected End Date
31 March 2016Completed Date
28 November 2016Description
Currently, in LMI-countries, there is a strong increment in the application of nuclear technologies, especially in the fields of electrical power/energy management, in the field of research, and in human health. For these countries, this implies an increase in nuclear power plants, more nuclear research facilities and more radiation facilities for medicine (radiation oncology and radiation diagnostics). In spite of strict regulations and safety measures, radiation accidents or unplanned radiation exposures may occur. Biological dosimetry implies different cytogentic assays and is used to estimate the absorbed dose in the exposed individual. Biodosimetry plays an important role in the triage and medical management of radiological casualties. The availability of national and regional biodosimetry programmes / laboratories will be very useful not only in the case of a nuclear disaster but also for radiation workers in environments with a certain radiation risk and for the general public. The major aim is to increase the preparedness of biological dosimetry laboratories / institutes in IAEA Member States (MSs) to react on radiation / nuclear accidents. The rationales are: to set up suitable standards to monitor individuals exposed to radiation, to update the existing technology, introduce “state of the art” technology and to initiate national, regional and international networks on biological dosimetry that can be extremely useful in scenarios of mass casualties.
Objectives
The major aim of the project is to increase the preparedness of biological dosimetry laboratories / institutes in IAEA-MSs to react on radiation / nuclear accidents nationally and in the region.
Specific Objectives
To complement and to add to already existing IAEA and WHO activities in this field; RANET (IAEA-Incidence and Emergency Unit, Nuclear safety); BiodoseNet (WHO).
To implement adequate technical information and expertise in LMI-countries to perform biological dosimetry and human risk assessment (i.e. in scenarios of environmental, occupational, clinical and accidental exposures to radiation of different qualities at low and high dose levels).
To initiate and give advice on different relevant research programs in order to enhance the current and future research programs to assess precisely the effect of ionizing radiations and human risk.
To monitor individuals exposed to radiation environmentally, occupationally, clinically as well as accidentally.
To unify/harmonize technically cytogenetic assays that are applicable for human biological dosimetry among different laboratories in the MSs, and by initiating different networks (national and international collaborations).
To update conventional biological assays and to implement state of the art technology in research centres having expertise on applying different types of cytogenetic assays for biological dosimetry immediately and/or retrospectively following (controlled and uncontrolled) exposure to radiation of different qualities.
Impact
The CRP had a major impact both on MSs and collaborating international organisations. The project straightened and harmonised biodosimetric services worldwide. It was performed in in a parallel to a number of TC capacity building national Biodosimetry projects including building biodosimetry lab in Lithuania (LIT6005, 2012-2015), which was featured as success story and Establishing Ukrainian Centre of Competence in biological dosimetry (UKR9034, 2016-2017). I served as a TO the first one and advised on the second. The CRP was a success, special issue of Genomic Integrity journal, consisted of 18 papers from participants and one editorial. This project led to a new CRP on Clinical application of Biodosimetry, which just started (2017-2021) and became very popular among MSs.
Relevance
The project is highly relevant to the project objectives and has significant Nuclear Component since it dealing with application biological radiation dosimetry methods to radiation emergencies,
CRP Publications
India
Book chapter
2014
Sharan, R.N., Nongrum, S., Keppen, J., Vaiphei, S.T., Kma, L. and Chaubey, R.C. (2014) Futureof Radiation Biodosimetry : Molecular Biomarker. In : Current Topics in Redox Biology, pp 61-81, Eds. Sharma, G. J. and Sharan, R.N., McGraw Hill Education
INS
Proceedings
2016
Syaifudin, M., S. Purnami, Y. Lusiyanti, and Z. Alatas, Establishment of a dose-response curve for X-ray-induced micronuclei in human lymphocytes. Genome Integrity, 2016. 7(1): p. 7-7.
RUS
Proceedings
2016
Neronova, E., Construction of calibration curve for premature chromosome condensation assay for dose assessment. Genome Integrity, 2016. 7(1): p. 9-9.
ROK
Proceedings
2016
Kim, C., C.-M. Kang, H. Kim, and H. Yun, Strong correlation among three biodosimetry techniques following exposures to ionizing radiation. Genome Integrity, 2016. 7(1): p. 11-11.
CPR
Proceedings
2016
Piao, C., J. Liu, J. Ruan, X. Su, and Y. Pan, Intercomparison in cytogenetic dosimetry among 22 laboratories in China. Genome Integrity, 2016. 7(1): p. 6-6.
IAEA
Special issue Vol 7(1) and 8(1)
2016/2017
Proceedings of the IAEA Coordinated Research Project (CRP) ‘Strengthening of “Biological dosimetry” in IAEA Member States: Improvement of current techniques and intensification of collaboration and networking among the different institutes’ (2012-2016) were published in the Special issue of the Genome Integrity (open access) journal, 2016-2017, Vol 7(1) and 8(1).
India
Patent application under ICT
2013
Provisional on 29th April 2013 (486/KOL/2013) and Final on 17th October 2014 (E-2/1772/2014/KOL) for “Radiation response proteins andradiation response genes and a technology of its quantitative measurement forbiodosimetry” (Applicants: Prof. R. N. Sharan and Secretary, DAE; Inventors: Prof. R. N.Sharan, Ms. S. Nongrum, Dr. S. T. Vaiphei, Dr. L. Kma and Dr. R. C. Chaubey).
Ukraine
Refereed journal paper
2013
Vinnikov, V.A. and N.A. Maznyk, Cytogenetic dose-response in vitro for biological dosimetry after exposure to high doses of gamma-rays. Radiat Prot Dosimetry, 2013. 154(2): p. 186-97
India
Refereed journal paper
2014
Vaiphei, S.T., Keppen, J.., Nongrum, S., Chaubey, R.C., Kma, L. and Sharan, R.N. (2014) Evaluation of endogenous control gene(s) for gene expression studies in human peripheral blood lymphocytes exposed to 60Co ?-radiation ex vivo, Journal of Radiation Research, pp 1–9.
LIT
Proceedings
2017
Vaisiunas, ?., A. Plieskiene, J. ?iliukas, K. Guogyte, O. Sevriukova, R. Ladygiene, and V. Janu?onis, Assessment of correlation between chromosomal radiosensitivity of peripheral blood lymphocytes after In vitro irradiation and normal tissue side effects for cancer patients undergoing radiotherapy. Genome Integrity, 2017. 8(1): p. 1-1.
ROK
Proceedings
2016
Kim, J., J.-H. Kim, and T. Ryu, Chromosomal aberrations in human peripheral blood lymphocytes after exposure to ionizing radiation. Genome Integrity, 2016. 7(1): p. 5-5.
International
Refereed journal paper
2013
Beinke, C., S. Barnard, H. Boulay-Greene, A. De Amicis, S. De Sanctis, F. Herodin, A. Jones, U. Kulka, F. Lista, D. Lloyd, P. Martigne, J. Moquet, U. Oestreicher, H. Romm, K. Rothkamm, M. Valente, V. Meineke, H. Braselmann, and M. Abend, Laboratory intercomparison of the dicentric chromosome analysis assay. Radiat Res, 2013. 180(2): p. 129-37
POL
Proceedings
217
Cebulska-Wasilewska, A., A. Stepien, G. Krajewska, M. Krzysiek, and P. Krajewski, Retrospective biological dosimetry at low and high doses of radiation and radioiodine impact on individual susceptibility to ionizing radiation. Genome Integrity, 2017. 8(1): p. 2-2.
PHI
Proceedings
2016
Asaad, A.-A., A.D. Asa, C. Asaad, G.J. Robles, G. Caraos, and M.L. Cobar, Enhancing cytogenetic biological dosimetry capabilities of the philippines for nuclear incident preparedness. Genome Integrity, 2016. 7(1): p. 4-4.
UKR
Proceedings
2017
Vinnikov, V., Optimizing the microscopy time schedule for chromosomal dosimetry of high-dose and partial-body irradiations. Genome Integrity, 2017. 8(1): p. 3-3.
IND
Proceedings
2017
Kashyap, E., J. Keppen, M. Ksoo, R. Sharan, S. Vaiphei, and S. Nongrum, Identification and preliminary validation of radiation response protein(s) in human blood for a high-throughput molecular biodosimetry technology for the future. Genome Integrity, 2017. 8(1): p. 5-5.
GEO
Proceedings
2016
Zedginidze, A., A. Kapanadze, D. Lomidze, E. Namchevadze, G. Ormocadze, and T. Nikuradze, Biodosimetry of persons chronically exposed to low and therapeutic doses of ionizing radiation. Genome Integrity, 2016. 7(1): p. 12-12.
International
Refereed journal paper
2013
Garcia, O., M. Di Giorgio, M.B. Vallerga, A. Radl, M.R. Taja, A. Seoane, J. De Luca, M. Stuck Oliveira, P. Valdivia, A.I. Lamadrid, J.E. Gonzalez, I. Romero, T. Mandina, G. Pantelias, G. Terzoudi, C. Guerrero-Carbajal, C. Arceo Maldonado, M. Espinoza, N. Oliveros, W. Martinez-Lopez, M.V. Di Tomaso, L. Mendez-Acuna, R. Puig, L. Roy, and J.F. Barquinero, Interlaboratory comparison of dicentric chromosome assay using electronically transmitted images. Radiat Prot Dosimetry, 2013. 154(1): p. 18-25
China
Refereed journal paper
2014
Chen, Y., P.K. Zhou, X.Q. Zhang, Z.D. Wang, Y. Wang, and F. Darroudi, Cytogenetic studies for a group of people living in Japan 1 year after the Fukushima nuclear accident. Radiat Prot Dosimetry, 2014. 159(1-4): p. 20-5
IAEA/SIN/IND
Proceedings
2016
Belyakov, O., P. Hande, and R. Sharan, Strengthening biological dosimetry in member states of the international atomic energy agency. Genome Integrity, 2016. 7(1): p. 1-1.
USA/SIN/IAEA
Proceedings
2017
Balajee, A., B. Banerjee, D. Zeegers, G. Low, M. Hande, M. Jayapal, N. Sundaram, O. Belyakov, P. Srivastava, R. Baskar, S. Venkatesan, S. Koh, and S. Sethu, Biomarkers of ionizing radiation exposure: A multiparametric approach. Genome Integrity, 2017. 8(1): p. 6-6.
URU
Proceedings
2017
Gregoire, E., M. Di Tomaso, and W. Martínez-López, Effects of valproic acid on radiation-induced chromosomal aberrations in human lymphocytes. Genome Integrity, 2017. 8(1): p. 4-4.
VIE
Proceedings
2016
Luyen, B., P. Duy, and T. Que, Calibration curve for dicentric chromosomes induced in human blood lymphocytes exposed to gamma rays at a dose rate of 12.5 mgy/s. Genome Integrity, 2016. 7(1): p. 2-2.
Japan
Refereed journal paper
2015
Suto et al.: Assessing the Applicability of FISH-based Prematurely Condensed Dicentric Chromosome Assay in Triage Biodosimetry. Health Physics, 2015 March,108(3): 371-376.
CAN
Proceedings
2017
Beaton-Green, L., M. Rodrigues, and R. Wilkins, The application of imaging flow cytometry to high-throughput biodosimetry. Genome Integrity, 2017. 8(1): p. 7-7.
Canada
Refereed journal paper
2013
Beaton, L.A., L. Marro, S. Samiee, S. Malone, S. Grimes, K. Malone, and R.C. Wilkins, Investigating chromosome damage using fluorescent in situ hybridization to identify biomarkers of radiosensitivity in prostate cancer patients. Int J Radiat Biol, 2013. 89(12): p. 1087-93
THA
Proceedings
2016
Rungsimaphorn, B., B. Rerkamnuaychoke, and W. Sudprasert, Establishment of dose-response curves for dicentrics and premature chromosome condensation for radiological emergency preparedness in Thailand. Genome Integrity, 2016. 7(1): p. 8-8.
Korea (ROK)
Refereed journal paper
2014
Tae Ho Ryu, Jin-Hong Kim and Jin Kyu Kim. Radiation exposure dose in human blood lymphocytes as assessed by the CBMN assay. J. Ecol. Environ. 37(4): 195-200, 2014
CPR
Proceedings
2016
Gao, G., J. Ruan, J. Liu, and Y. Pan, Study on ?H2AX expression of lymphocytes as a biomarker in radiation biodosimetry. Genome Integrity, 2016. 7(1): p. 10-10.
France
Refereed journal paper
2012
Herodin, F., S. Richard, N. Grenier, P. Arvers, P. Gerome, S. Bauge, J. Denis, H. Chaussard, S. Gouard, J.F. Mayol, D. Agay, and M. Drouet, Assessment of total- and partial-body irradiation in a baboon model: preliminary results of a kinetic study including clinical, physical, and biological parameters. Health Phys, 2012. 103(2): p. 143-9
GNA
Proceedings
2016
Agyekum, A., A. Serafin, D. Achel, E. Achoribo, F. Akuamoa, J. Yarney, J. Akudugu, K. Kyei, N. Adu-Bobi, R. Adaboro, S. Tagoe, S. Agbenyegah, and S. Donkor, Towards establishing capacity for biological dosimetry at ghana atomic energy commission. Genome Integrity, 2016. 7(1): p. 3-3.
China
Refereed journal paper
2014
Wang, Z., H. Hu, M. Hu, X. Zhang, Q. Wang, Y. Qiao, H. Liu, L. Shen, P. Zhou, and Y. Chen, Ratio of gamma-H2AX level in lymphocytes to that in granulocytes detected using flow cytometry as a potential biodosimeter for radiation exposure. Radiat Environ Biophys, 2014. 53(2): p. 283-90
Cuba
Refereed journal paper
2015
Romero, I., A.I. Lamadrid, J.E. Gonzalez, O. Garcia, P. Voisin, and L. Roy, Shortening the culture time in cytogenetic dosimetry using PCC-R assay. Radiat Prot Dosimetry, 2015. 163(4): p. 424-9
IAEA
Poster presentation
2014
Oleg V. Belyakov, Jan Wondergem, and Eduardo Rosenblatt. Strengthening of biological dosimetry in IAEA member states related to the CRP E35008, poster presentation at the 60th Annual Meeting of Radiation Research Society, 21-24 September 2014, Las Vegas, NV, USA.
Japan
Refereed journal paper
2013
Suto, Y., M. Hirai, M. Akiyama, G. Kobashi, M. Itokawa, M. Akashi, and N. Sugiura, Biodosimetry of restoration workers for the Tokyo Electric Power Company (TEPCO) Fukushima Daiichi nuclear power station accident. Health Phys, 2013. 105(4): p. 366-73