Effects of Mutagenic Agents on the DNA Sequence in Plants
Closed for Proposals
Project Type
Coordinated Research ProjectProject Code
D24011
CRP
567
Approved Date
3 September 2003Project Status
ClosedStart Date
15 September 2003Expected End Date
22 March 2010Completed Date
22 March 2010Description
Physical, chemical and biological mutagenic agents cause genes to mutate at rates above the spontaneous baseline, thus producing a range of novel traits and broadening the genetic diversity of plants. The use of induced mutants in breeding has had a profound impact on world agriculture and more than 2300 new crop varieties, all carrying novel induced variation, have now been officially registered (IAEA Mutant Variety Database). This has all been achieved largely in the absence of knowledge of the precise changes induced at the DNA level. Indeed there still is very little understanding of the nature of the mutations induced by different mutagens. With the advent of molecular genetics and genomics, induced mutations are finding new applications in modern plant breeding. Reverse genetics and deletion library methodologies capable of discovering new genes and their modes of action are often underpinned by variation induced by both physical and chemical mutagens. However the efficiency of these new methods will be enhanced only when the type, frequency and distribution of mutations in a range of crop species can be predicted, and ideally directed.
Today, with the range of technologies available to the scientific community to assay variation in DNA sequence and the availability of a vast amount of crop plant DNA sequence, including the complete sequences of both Arabidopsis and rice, these questions can now be approached. This Coordinated Research Project sets out to define the type, frequency and patterns of molecular changes induced by the range of physical and chemical mutagens in a range of crop species.
Objectives
This Coordinated Research Project aimed to understand the mechanism of mutation induction in plants and to quantify the types (base pair changes or deletions), frequencies (rates of change relative to mutagens dose) and patterns (induction of changes in different parts of the genome) of changes in DNA induced by a range of physical and chemical mutagens in a range of key crop plant species. Knowledge obtained is bound to assist Member States in enhancing crop breeding programmes through the application of targeted induced mutations, complementary genomic approaches and knowledge for the identification and selection of mutants of specific genes with the objective of increasing agricultural sustainability, food security and economic stability.
Specific Objectives
1) Measure the microscopic characteristics of the spontaneous and induced mutation process at the molecular level using nucleic acid scanning methodologies at the whole genome/transcriptome level and mutation trap assays.
2) Determine the existence of mutation-induced heterogeneities.
3) Test if mutations are constrained by the structure of nucleic acids.
4) Assay the effect of different doses of physical and chemical mutagens
5) Compare different physical and chemical mutagens at equivalent doses
6) Develop a knowledge base for enlightened use and application of mutation induction, including the descriptions and definitions of
a) levels of DNA damage
b) effects of repair systems in plants following mutagenic treatment
c) types, frequencies, rates and patterns of molecular change
induced by the range of physical and chemical mutagens for use in crop plant breeding,
relative to background spontaneous mutation.
7) An up-to-date review of the present understanding of the mechanisms involved in and the effects of mutation induction in plants
Impact
? Enhancement of capacity in techniques to generate and analyse mutant germplasm in developing countries .
? Guidance tools on the use of appropriate mutagens to achieve mutation breeding objectives developed.
? Development of a repository of knowledge on mutagens and their efficacy.
? Two rice varieties suitable for diabetics and obesity patients commercialized.
? Mutant lines tolerant to postharvest physiological deterioration (PPD) in cassava identified.
? Drought tolerant soybean variants characterized
Relevance
1) Provide guidelines to Member States for the informed application of induced mutation to crop improvement;
2) Characterize DNA damage, repair mechanisms and types of lesions in plants induced by treatment with physical and chemical mutagens.
3) Promote efficient and safer crop improvement by defining thresholds for environmental protection;
4) Produce and communicate research results that enhance the quality and relevance of mutation breeding;
5) Foster implementation regional collaboration, knowledge transfer, technology, and genetic and molecular resources between all participating research groups for their mutual benefit;
6) To produce guidelines and publish methods on the application induced mutations to various crops.
7) Contribute to the development of enhanced germplasm with traits that are essential for crop breeding programmes in developing countries;
CRP Publications
Bulgaria
scientific peer reviewed
2009
Gecheff K., V. Manova, G. Bonchev, M. Kitanova, M. Vlahova, L. Stoilov. (2009) Position-specific effects in the action of mutagenic agents on the chromosomes of barley (Hordeum vulgare L.) Genetics and Breeding, in press.
Korea
scientific peer reviewed
2008
Janiak A, Kim MY, Van K, Lee S-H.(2008) Application of degenerate oligonucleotide primed PCR (DOP-PCR) for SNP discovery in soybean. Euphytica,162:249-256.
China
scientific peer reviewed
2006
Wu W, Liu X, Shu XL, Wu DX. (2006) Progress in the study of resistant starch and functional rice for prevention of diabetes and hyperlipidemia. Journal of Nuclear Agricultural Science, 20:60-63.
USA
scientific peer reviewed
2007
Caetano-Anollés, G. (2007) Evolution of genome size in the grasses. Crop Science 45: 1809-1816
scientific (Agency) peer reviewed
2007
Guo, H.J, L.X. Liu, W.B. Han, S.R. Zhao, L. S. Zhao, L. Sui, K. Zhao, F.Q. Kong, J. Wang. Biological effects of high energy 7li ion beams implantation on wheat. Plant Mutation Reports, 2007,1(3):31-35.
USA
scientific peer reviewed
2007
Martin N, EA Ruedi, R LeDuc, F-J Sun and G Caetano-Anollés (2007) Geneinterleaving patterns of synteny in the Saccharomyces cerevisiae genome: are they proof of an ancient genome duplication event? Biology Direct 2: 23.
USA
scientific peer reviewed
2007
Ceballos, H., Sánchez, T.; Morante, N.; Fregene, M.; Dufour, D.; Smith, A.M.; Denyer, K.; Pérez, J.C.; Calle, F.; Mestres, C. Discovery of an amylose-free starch mutant in cassava (Manihot esculenta Crantz). Journal of Agricultural and Food Chemistry 2007, 55(18), 7469-7476.
China
scientific peer reviewed
2007
Jia Limeng, Zhang Zhizhuan, Shu Xiaoli, Li Chengdao, Wu Dianxing. (2007) Starch properties and structure of a wheat mutant high in resistant starch. The Open Agriculture Journal, 2007, 1:5-10.
China
scientific (national) peer reviewed
2005
Liu Luxiang, Guo Huijun, Zhao Linshu, Zhao Kui, Zhao Shirong, Sui Li, Kong Fuquan.Bio-effects of 7Li ion beams irradiation on wheat, 2005 Annual report for Beijing National Tandem Accelerator Laboratory, Beijing: Atomic Energy Press, 2007, p104-105
China
scientific peer reviewed
2006
Yang CZ, Shu XL, Zhang LL, Wang XY, Zhao HJ, Ma CX, Wu DX. (2006) Starch properties of mutant rice high in resistant starch. Journal of Agricultural and Food Chemistry, 54: 523-528.
2007
Scientific paper at international conferences and symposia
Ceballos, H., T. Sánchez, N. A. P. Tofin?o, A. Rosero, D. Doufor, A. M. Smith, K. Danyer, J.C. Pérez, N. Morante, F. Calle, Z. Lentini, M. Fregene and C. Mestres. (2007). Development and identification of cassava clones with special starch characteristics. Proceedings of the Starch Update Conference. Bangkok, Thailand. November 6-7.
China
scientific peer reviewed
2008
YANG Song-rui, LIU Lu-xiang, ZHAO Lin-shu. (2008) Current status and prospective of crop improvement for low phytic acid content, Journal of Plant Genetic Resources, 9: 263-265.
China
scientific peer reviewed
2007
Xin Qing-guo, Liu Lu-xiang, Guo Hui-jun, Zhao Lin-shu, Yu Yuan-jie , Zhao Kui, Sui Li, Kong Fu-quan, Zhao Shi-rong. (2007) SSR Analysis of M1 generation variation of dry seeds implanted by 7Li ion beam in wheat, Journal of Triticeae Crops, 27:560-564.
China
scientific peer reviewed
2006
Shu XL, Jiao GA, Fitzgerald M, Yang CZ, Shu QY, Wu DX. (2006) Starch structure and digestibility of rice high in resistant starch. Starch, 58: 411-417.
Bulgaria
Scientific paper at international conferences and symposia
2008
Manova, V., M. Georgieva, B. Borisov, B. Stoilova, K. Gecheff and L. Stoilov Genomic and gene-specific induction and repair of DNA damage in barley. Invited lecture IAEA-CN-167-056 FAO/IAEA International Symposium on Induced Mutations in Plants, Vienna, Austria.
China
Scientific paper at international conferences and symposia
2005
Zhao Linshu,Liu Luxiang,Guo Huijun,et al. (2005). Production of mutants with high inorganic phosphorus content in seeds by germplasm reselection and mutation techniques in wheat. Paper Abstracts Collections of National Workshop on Crop Biotechnology and Mutation Techniques, Nov 7-9, 2005, Fujian Prov. p130.
China
book
2009
Wu, DX and Shu XL (eds). (2009) Rice starch quality research and utilization. China Agriculture Publishing House.
USA
scientific peer reviewed
2008
C. A. Cullis, B. J. Vorster, C. Van Der Vyver and K. J. Kunert. (2008) Transfer of Genetic Material Between the Chloroplast and Nucleus: How is it Related to Stress in Plants? Annals of Botany doi: 10.1093/aob/mcn173.
Bulgaria
scientific peer reviewed
2008
Georgieva M. and L. Stoilov (2008) Assessment of DNA strand breaks induced by bleomycin in barley by the comet assay. Environmental and Molecular Mutagenesis, 49, 381-387.
China
scientific (national) peer reviewed
2006
Han W B, Liu L X, Guo H J, Zhao L S, Li J C, Zhang S P, Wang C S, Zhao S R. SSR analysis on M1 variation of wheat irradiated by mixed high energy particle field. Acta Agriculture Nucleatae Sinica, 2006, 20: 165-168.
China
scientific (national) peer reviewed
2004
Liu L. X,, Wang J Zhao L.Sh, et al. (2004). Research progress in mutational effects of aerospace on crop and ground simulation on aerospace environment factors, Acta Agriculturae Nucleatae Sinica, 18:247-251.
China
book
2006
Wu, DX (ed). (2006) Functional rice research and utilization. China Metrology Publishing House.
China
Scientific paper at international conferences and symposia
2005
Guo Huijun,Liu Luxiang,Zhao Linshu (2005).DNA damages induced by mixed particle field irradiation . Paper Abstracts Collections of National Workshop on Crop Biotechnology and Mutation Techniques, Nov 7-9, 2005, Fujian Prov. p198.
Bulgaria
scientific peer reviewed
2009
Dimitrova A., E. Todorovska, N. Christov, L. Stoilov, A. Atanassov and K. Gechef. (2009) Molecular characterization of ionizing radiation-induced mutational alterations in barley. Genetics and Breeding, in press.
China
scientific peer reviewed
2008
HU Yan-ling, LIU Lu-xiang, GUO Hui-jun, ZHAO Lin-shu, ZHAO Shi-rong, SSR analysis of dwarf mutants derived from mixed particle field irradiation in wheat. Journal of Nuclear Agricultural Sciences, 2008, 22(4): 399-403.
Columbia
scientific peer reviewed
2008
Ceballos, H., T. Sánchez, K. Denyer, A. P. Tofi?o, E. A. Rosero, D. Dufour, A. Smith, N. Morante, J. C. Pérez, and B. Fahy. (2008). Induction and identification of a small-granule, high-amylose mutant in cassava (Manihot esculenta Crantz). Journal of Agricultural and Food Chemistry 56(16): 7215-7222
Bulgaria
scientific peer reviewed
2006
Manova, V., K. Gecheff and L. Stoilov (2006) Efficient repair of bleomycin-induced double-strand breaks in barley ribosomal genes, Mutation Research, 601, 179-190.
USA
scientific peer reviewed
2008
Ceballos, H., T. Sánchez, K. Denyer, A. P. Tofi?o, E. A. Rosero, D. Dufour, A. Smith, N. Morante, J. C. Pérez, and B. Fahy. (2008). Induction and identification of a small-granule, high-amylose mutant in cassava (Manihot esculenta Crantz). Journal of Agricultural and Food Chemistry 56(16): 7215-7222
China
scientific peer reviewed
2007
Shu XL, Gao JK, Jia LM, Song YL, Wu DX. (2007) The influence of chain length of amylopectin on resistant starch in rice (Oryza sativa L.). Starch, 59: 504-509.
Korea
book chapter
2007
Van K, Kim MY, Lee S-H. (2007) Genomics of root nodulation in soybean. In Genomics Assisted Crop Improvement, Vol 2. Genomics Applications in Crops Edited by: Varshney RK, Tuberosa R. Springer. pp425-442.
China
scientific peer reviewed
2006
Zuo XX, Zheng T, Shu XL, Shu QY, Wu DX. (2006) Advances in screening and characterization study of rice endosperm mutants. Journal of Nuclear Agricultural Science, 20:118-122.
China
scientific peer reviewed
2005
Liu Luxiang, Zhao Linshu, Guo Huijun et al (2005). Study on mutagenic effects of mixed particle field irradiation in winter wheat. Science Technology and Engineering, 22:1641-1644
Columbia
Scientific paper at international conferences and symposia
2008
Sanchez, T., A. Rosero, A.P. Tofi?o, K. Denyer, A. Smith, H. Ceballos, N. Morante, and J.C. Pérez. (2008). Induction and identification of useful mutations for root quality traits in cassava. FAO/IAEA International Symposium on Induced Mutations in Plants. 12-15 August, Vienna, Austria. p. 107.
China
scientific (national) peer reviewed
2005
Liu Luxiang,Han Weibo, Guo Huijun, et al (2005). Cytological effects of mixed particle field irradiation in wheat. Acta Agriculturae Nucleatae Sinica, 19:1-5.
Korea
scientific peer reviewed
2008
Van K, Jang HJ, Jang Y-E, Lee S-H. (2008) Regeneration of plants from EMS-treated immature embryo cultures in soybean [Glycine max (L.) Merr.]. J Crop Sci Biotechnol., 11:119-126.
China
Scientific paper at international conferences and symposia
2005
Guo Huijun, Liu Luxiang, Zhao Shirong et al (2005) .Biological effects of 43MeV 7Li ion beam implantation in wheat Paper Abstracts Collections of National Workshop on Crop Biotechnology and Mutation Techniques, Nov 7-9, 2005, Fujian Prov. p163.
Bulgaria
Scientific paper at international conferences and symposia
2008
Manova, V., M. Georgieva, B. Borisov, B. Stoilova, K. Gecheff and L. Stoilov (2008) Genomic and gene-specific induction and repair of DNA damage in barley. Invited lecture IAEA-CN-167-056 FAO/IAEA International Symposium on Induced Mutations in Plants, Vienna, Austria.
Bulgaria
scientific (national) peer reviewed
2004
Dimitrova A., L. Stoilov and K. Gecheff. (2004) Loop organization of ribosomal DNA in barley. Compt. Rend. Acad. Bulg. Sci., 57, 57-60.
Korea
scientific peer reviewed
2004
Van K, Hwang E.-Y, Kim MY, Kim Y.-H, Cho YI, Cregan PB, Lee S.-H. (2004) Discovery of single nucleotide polymorphisms in soybean using primers designed from ESTs. Euphytica,139:147-157.
India
scientific peer reviewed
2006
Kavitha M, Bauer P, Sharada MS, Al-Hammadi AS, Sharma R (2006) Genetic characterization of the polycotyledon locus in tomato. Theoretical and Applied Genetics 113: 673-683.
Bulgaria
Scientific paper at international conferences and symposia
2008
Dimitrova A., E. Todorovska, N. Hristov, K. Gecheff, Stoilov L. and A. Atanassov (2008) Molecular analysis of ionizing radiation induced genetic variability in barley. Poster presentation IAEA-CN-167-076, FAO/IAEA International Symposium on Induced Mutations in Plants, Vienna, Austria.
Bulgaria
scientific peer reviewed
2006
Gecheff K. I. (2006) Inversion-derived cytological marking of the chromosome complement of barley (Hordeum vulgare L.). Genetics and Breeding, 35, 19-24.
Bulgaria
scientific peer reviewed
2006
Stoilova Ts., A. Dimitrova, Ts. Angelova and K. Gecheff (2006). Assessment of the genetic diversity in barley mutant lines using biochemical markers. Genetics and Breeding, 35, 3-10.
Bulgaris
Scientific paper at international conferences and symposia
2008
Gecheff, K., L. Stoilov, V. Manova and G. Bonchev (2008) Position-specific effects in the action of mutagenic agents on the chromosomes of barley (Hordeum vulgare L.) Poster presentation IAEA-CN-167-046, FAO/IAEA International Symposium on Induced Mutations in Plants, Vienna, Austria.
China
scientific (national) peer reviewed
2008
GUO Hui-jun, LIU Lu-xiang, HAN Wei-bo, ZHAO Shi-rong, LI Jia-cai, ZHAO Lin-shu, WANG Jing, Mutagenic Effects of Mixed Particle Field Irradiation on Wheat. Scientia Agricultura Sinica, 2008, 41(3): 654-660.
Korea
scientific peer reviewed
2005
Kim MY, Van K, Lestari P, Moon J-K, Lee S-H. (2005) SNP identification and SNAP marker development for a GmNARK gene controlling supernodulation in soybean. Theor Appl Genet.,110:1003-1010.
Korea
scientific (national) peer reviewed
2005
Cai CM, Van K, Kim MY, Lee S-H. (2005) Optimization of SNP genotyping assay with fluorescence polarization detection. Kor J Crop Sci., 50:361-367.
Bulgaria
Scientific paper at international conferences and symposia
2008
Georgieva M., V. Manova, L. Liu and L. Stoilov (2008) Induction of DNA damage by Li-ions and gamma rays in barley genome. Poster presentation IAEA-CN-167-075, FAO/IAEA International Symposium on Induced Mutations in Plants, Vienna, Austria.
Bulgaria
Scientific paper at international conferences and symposia
2008
Stoilov L. and K.Gecheff (2008) Restriction endonucleases as a tool for in vivo induction of chromosomal and DNA damage in barley genome. Invited lecture IAEA-CN-167-041 FAO/IAEA International Symposium on Induced Mutations in Plants, Vienna, Austria.
Bulgaria
Scientific paper at international conferences and symposia
2005
Manova V., Gecheff K. and Stoilov L. (2005) Induction and repair of bleomycin-induced DNA damage in barley genome- Abstract, Keystone Symposia “Genome Instability and Repair (C2)”, Sagebrush Inn and Conference Center, New Mexico, USA.
China
scientific (national) peer reviewed
2007
Liu Lu-xiang, Zhao Lin-shu, Guo Hui-jun. (2007) Current status and perspective outlook of space induced mutation breeding in crop plants, Review of China Agricultural Science and Technology, 9:26-29.
Columbia
book chapter
2007
Ceballos, H., M. Fregene, J. C. Pérez, N. Morante and F. Calle. Cassava Genetic Improvement (2007). In: Breeding Major Food Staples (M.S. Kang and P.M. Priyadarshan eds.). p. 365-391, Blackwell Publishing. Ames, IA. USA
Bulgaria
scientific peer reviewed
2005
Manova V. and L. Stoilov (2005). Repair of DNA double-strand breaks in eukaryotic genome. Genetics and Breeding, 34, 39-61.